Phenotypic characteristics of joint fluid cells from patients with continuous joint effusion after total knee arthroplasty

Yasuo Niki, Hideo Matsumoto, Toshiro Otani, Taku Yatabe, Atsushi Funayama, Shinichi Maeno, Taisuke Tomatsu, Yoshiaki Toyama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Joint effusion after total joint arthroplasty (TJA) is a manifestation of inflammatory reactions within the prosthetic joint. Among the various causes for joint effusion following TJA, deep infection (DI), wear particle-induced synovitis (PS) and metal sensitivity to the implant should be excluded as soon as possible, as these may result in the failure of TJA. The present study analyzed joint fluid cells from patients after total knee arthroplasty (TKA) using fluorescence-activated cell sorter (FACS), and examined the feasibility of using FACS to exclude the possibility of biomaterial-related complication. A total of 72 TKAs from 64 patients suffering from joint effusion were examined in this study. Joint fluid was aspirated in outpatient clinics and applied to FACS. The results indicated that patients could be clearly classified into four types based on forward/side scatter profiles. Analysis of specific CD markers revealed that leukocytes were selectively recruited from blood to inflamed prosthetic joints. Dominant cell types were CD16+neutrophils in DI and increased rheumatoid activity, CD14+macrophages in PS, and CD3+CD45RO+T cells in metal sensitivity. These findings suggest the feasibility of diagnosing joint effusion by analyzing dominant cell type recruited using FACS. In conclusion, FACS may offer a useful tool for analyzing joint fluid cells from post-TJA patients and for excluding biomaterial-related complication following TJA.

Original languageEnglish
Pages (from-to)1558-1565
Number of pages8
JournalBiomaterials
Volume27
Issue number8
DOIs
Publication statusPublished - 2006 Mar

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Arthroplasty
Knee Replacement Arthroplasties
Joints
Fluorescence
Fluids
Biocompatible Materials
Prosthetics
Biomaterials
Metals
T-cells
Macrophages
Synovitis
Blood
Wear of materials
Ambulatory Care Facilities
Infection

Keywords

  • Biomaterial-related complication
  • Joint effusion
  • Joint fluid cell
  • Total knee arthroplasty

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Phenotypic characteristics of joint fluid cells from patients with continuous joint effusion after total knee arthroplasty. / Niki, Yasuo; Matsumoto, Hideo; Otani, Toshiro; Yatabe, Taku; Funayama, Atsushi; Maeno, Shinichi; Tomatsu, Taisuke; Toyama, Yoshiaki.

In: Biomaterials, Vol. 27, No. 8, 03.2006, p. 1558-1565.

Research output: Contribution to journalArticle

Niki, Yasuo ; Matsumoto, Hideo ; Otani, Toshiro ; Yatabe, Taku ; Funayama, Atsushi ; Maeno, Shinichi ; Tomatsu, Taisuke ; Toyama, Yoshiaki. / Phenotypic characteristics of joint fluid cells from patients with continuous joint effusion after total knee arthroplasty. In: Biomaterials. 2006 ; Vol. 27, No. 8. pp. 1558-1565.
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