Phenotypic study after cultivated limbal epithelial transplantation for limbal stem cell deficiency

Motoko Kawashima, Tetsuya Kawakita, Yoshiyuki Satake, Kazunari Higa, Jun Shimazaki

Research output: Contribution to journalArticle

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Abstract

Objective: To report phenotypic analysis of epithelia before and following cultivated limbal epithelial transplantation (CLET) for eyes with limbal stem cell deficiency. Methods: Six patients with limbal stem cell deficiency (3 with alkali burns, 2 with Stevens-Johnson syndrome, and 1 with pseudo-ocular cicatricial pemphigoid) were subjected to CLET and subsequent keratoplasty. Immunohistochemical analysis for cytokeratin 3, cytokeratin 13, MUC5AC (Mucin 5 subtype AC), and α smooth muscle actin was performed in specimens obtained at CLET and keratoplasty. Clinical outcome was assessed according to epithelial phenotype, visual acuity, neovascularization, and graft clarity. Results: Secondary keratoplasty was performed following CLET during a mean interval of 6.8 months. Postoperative visual acuity improved by more than 2 lines over a mean ± SD observation period of 25.1 ± 13.2 months following keratoplasty, with reduction of neovascularization. Phenotypic study revealed that epithelia were positive for cytokeratin 13 and MUC5AC, but negative for cytokeratin 3, with α smooth muscle actin-positive cells in the stroma in all patients before CLET. After CLET, 4 eyes showed positive immunostaining to cytokeratin 3 but negative immunostaining to cytokeratin 13 and MUC5AC, with no α smooth muscle actin-positive cells. Conclusion: Cultivated limbal epithelial transplantation is a useful approach in the treatment of limbal stem cell deficiency, restoring a feasible microenvironment in the ocular surface and securing a corneal epithelial phenotype.

Original languageEnglish
Pages (from-to)1337-1344
Number of pages8
JournalArchives of Ophthalmology
Volume125
Issue number10
DOIs
Publication statusPublished - 2007 Oct
Externally publishedYes

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Stem Cell Transplantation
Transplantation
Keratin-3
Keratin-13
Corneal Transplantation
Mucins
Smooth Muscle
Actins
Stem Cells
Visual Acuity
Epithelium
Benign Mucous Membrane Pemphigoid
Phenotype
Stevens-Johnson Syndrome
Alkalies
Burns
Observation
Transplants

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Phenotypic study after cultivated limbal epithelial transplantation for limbal stem cell deficiency. / Kawashima, Motoko; Kawakita, Tetsuya; Satake, Yoshiyuki; Higa, Kazunari; Shimazaki, Jun.

In: Archives of Ophthalmology, Vol. 125, No. 10, 10.2007, p. 1337-1344.

Research output: Contribution to journalArticle

Kawashima, Motoko ; Kawakita, Tetsuya ; Satake, Yoshiyuki ; Higa, Kazunari ; Shimazaki, Jun. / Phenotypic study after cultivated limbal epithelial transplantation for limbal stem cell deficiency. In: Archives of Ophthalmology. 2007 ; Vol. 125, No. 10. pp. 1337-1344.
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