Phenylethanoid glycosides targeting mammalian DNA polymerases

Akira Iida; Fumiyuki Kiuchi; Michiho Ito; Gisho Honda; Yoshiyuki Mizushina; Horomi Yoshida; Kanat Sarsenbaev

Phenylethanoid glycosides targeting mammalian DNA polymerases

Akira Iida, Fumiyuki Kiuchi, Michiho Ito, Gisho Honda, Yoshiyuki Mizushina, Horomi Yoshida, Kanat Sarsenbaev

Research output: Contribution to journal › Article › peer-review

Abstract

In our continuing search to discover bioactive compounds from medicinal plants, a methanolic extract of Verbascum songaricum collected in Kazakhstan was investigated. Activity-guided fractionation of the extract prepared from the plant, using a sensitive assay to monitor DNA polymerase reactions, resulted in the isolation of two known phenylethanoid glycosides, poliumoside (1) and verbascoside (2). Glycoside 1 was shown to inhibit mammalian DNA polymerases α, β, δ and ε with IC₅₀ values of 42, 23, 35 and 9 [μM, respectively, while those of glycoside 2 were all more than 100 [μM.

Original language	English
Pages (from-to)	146-149
Number of pages	4
Journal	Natural Medicines
Volume	57
Issue number	4
Publication status	Published - 2003 Aug
Externally published	Yes

Keywords

DNA polymerase inhibition
Poliumoside
Verbascoside, phenylethanoid glycoside
Verbascum songaricum

ASJC Scopus subject areas

Molecular Medicine

Cite this

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title = "Phenylethanoid glycosides targeting mammalian DNA polymerases",

abstract = "In our continuing search to discover bioactive compounds from medicinal plants, a methanolic extract of Verbascum songaricum collected in Kazakhstan was investigated. Activity-guided fractionation of the extract prepared from the plant, using a sensitive assay to monitor DNA polymerase reactions, resulted in the isolation of two known phenylethanoid glycosides, poliumoside (1) and verbascoside (2). Glycoside 1 was shown to inhibit mammalian DNA polymerases α, β, δ and ε with IC50 values of 42, 23, 35 and 9 [μM, respectively, while those of glycoside 2 were all more than 100 [μM.",

keywords = "DNA polymerase inhibition, Poliumoside, Verbascoside, phenylethanoid glycoside, Verbascum songaricum",

author = "Akira Iida and Fumiyuki Kiuchi and Michiho Ito and Gisho Honda and Yoshiyuki Mizushina and Horomi Yoshida and Kanat Sarsenbaev",

year = "2003",

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language = "English",

volume = "57",

pages = "146--149",

journal = "Journal of Natural Medicines",

issn = "1861-0293",

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TY - JOUR

T1 - Phenylethanoid glycosides targeting mammalian DNA polymerases

AU - Iida, Akira

AU - Kiuchi, Fumiyuki

AU - Ito, Michiho

AU - Honda, Gisho

AU - Mizushina, Yoshiyuki

AU - Yoshida, Horomi

AU - Sarsenbaev, Kanat

PY - 2003/8

Y1 - 2003/8

N2 - In our continuing search to discover bioactive compounds from medicinal plants, a methanolic extract of Verbascum songaricum collected in Kazakhstan was investigated. Activity-guided fractionation of the extract prepared from the plant, using a sensitive assay to monitor DNA polymerase reactions, resulted in the isolation of two known phenylethanoid glycosides, poliumoside (1) and verbascoside (2). Glycoside 1 was shown to inhibit mammalian DNA polymerases α, β, δ and ε with IC50 values of 42, 23, 35 and 9 [μM, respectively, while those of glycoside 2 were all more than 100 [μM.

AB - In our continuing search to discover bioactive compounds from medicinal plants, a methanolic extract of Verbascum songaricum collected in Kazakhstan was investigated. Activity-guided fractionation of the extract prepared from the plant, using a sensitive assay to monitor DNA polymerase reactions, resulted in the isolation of two known phenylethanoid glycosides, poliumoside (1) and verbascoside (2). Glycoside 1 was shown to inhibit mammalian DNA polymerases α, β, δ and ε with IC50 values of 42, 23, 35 and 9 [μM, respectively, while those of glycoside 2 were all more than 100 [μM.

KW - DNA polymerase inhibition

KW - Poliumoside

KW - Verbascoside, phenylethanoid glycoside

KW - Verbascum songaricum

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AN - SCOPUS:0042973157

SN - 1861-0293

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JF - Journal of Natural Medicines

IS - 4

ER -

Phenylethanoid glycosides targeting mammalian DNA polymerases

Abstract

Keywords

ASJC Scopus subject areas

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