Phenylethanoid glycosides targeting mammalian DNA polymerases

Akira Iida, Fumiyuki Kiuchi, Michiho Ito, Gisho Honda, Yoshiyuki Mizushina, Horomi Yoshida, Kanat Sarsenbaev

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In our continuing search to discover bioactive compounds from medicinal plants, a methanolic extract of Verbascum songaricum collected in Kazakhstan was investigated. Activity-guided fractionation of the extract prepared from the plant, using a sensitive assay to monitor DNA polymerase reactions, resulted in the isolation of two known phenylethanoid glycosides, poliumoside (1) and verbascoside (2). Glycoside 1 was shown to inhibit mammalian DNA polymerases α, β, δ and ε with IC50 values of 42, 23, 35 and 9 [μM, respectively, while those of glycoside 2 were all more than 100 [μM.

Original languageEnglish
Pages (from-to)146-149
Number of pages4
JournalNatural Medicines
Volume57
Issue number4
Publication statusPublished - 2003 Aug
Externally publishedYes

Fingerprint

DNA-Directed DNA Polymerase
Glycosides
Verbascum
Kazakhstan
Plant Extracts
Medicinal Plants
Fractionation
Inhibitory Concentration 50
Assays

Keywords

  • DNA polymerase inhibition
  • Poliumoside
  • Verbascoside, phenylethanoid glycoside
  • Verbascum songaricum

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Iida, A., Kiuchi, F., Ito, M., Honda, G., Mizushina, Y., Yoshida, H., & Sarsenbaev, K. (2003). Phenylethanoid glycosides targeting mammalian DNA polymerases. Natural Medicines, 57(4), 146-149.

Phenylethanoid glycosides targeting mammalian DNA polymerases. / Iida, Akira; Kiuchi, Fumiyuki; Ito, Michiho; Honda, Gisho; Mizushina, Yoshiyuki; Yoshida, Horomi; Sarsenbaev, Kanat.

In: Natural Medicines, Vol. 57, No. 4, 08.2003, p. 146-149.

Research output: Contribution to journalArticle

Iida, A, Kiuchi, F, Ito, M, Honda, G, Mizushina, Y, Yoshida, H & Sarsenbaev, K 2003, 'Phenylethanoid glycosides targeting mammalian DNA polymerases', Natural Medicines, vol. 57, no. 4, pp. 146-149.
Iida A, Kiuchi F, Ito M, Honda G, Mizushina Y, Yoshida H et al. Phenylethanoid glycosides targeting mammalian DNA polymerases. Natural Medicines. 2003 Aug;57(4):146-149.
Iida, Akira ; Kiuchi, Fumiyuki ; Ito, Michiho ; Honda, Gisho ; Mizushina, Yoshiyuki ; Yoshida, Horomi ; Sarsenbaev, Kanat. / Phenylethanoid glycosides targeting mammalian DNA polymerases. In: Natural Medicines. 2003 ; Vol. 57, No. 4. pp. 146-149.
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