Pho85 kinase, a cyclin-dependent kinase, regulates nuclear accumulation of the Rim101 transcription factor in the stress response of Saccharomyces cerevisiae

Masafumi Nishizawa, Mirai Tanigawa, Michio Hayashi, Tatsuya Maeda, Yoshiaki Yazaki, Yasushi Saeki, Akio Toh-e

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The budding yeast Saccharomyces cerevisiae alters its gene expression profile in response to changing environmental conditions. The Pho85 kinase, one of the yeast cyclin-dependent kinases (CDK), is known to play an important role in the cellular response to alterations in parameters such as nutrient levels and salinity. Several genes whose expression is regulated, either directly or indirectly, by the Rim101 transcription factor become constitutively activated when Pho85 function is absent,. Because Rim101 is responsible for adaptation to alkaline conditions, this observation suggests an interaction between Pho85 and Rim101 in the response to alkaline stress. We have found that Pho85 affects neither RIM101 transcription, the proteolytic processing that is required for Rim101 activation, nor Rim101 stability. Rather, Pho85 regulates the nuclear accumulation of active Rim101, possibly via phosphorylation. Additionally, we report that Pho85 and the transcription factor Pho4 are necessary for adaptation to alkaline conditions and that PTK2 activation by Pho4 is involved in this process. These findings illustrate novel roles for the regulators of the PHO system when yeast cells cope with various environmental stresses potentially threatening their survival.

Original languageEnglish
Pages (from-to)943-951
Number of pages9
JournalEukaryotic Cell
Volume9
Issue number6
DOIs
Publication statusPublished - 2010 Jun

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Cyclin-Dependent Kinases
Saccharomyces cerevisiae
Transcription Factors
Phosphotransferases
Yeasts
Saccharomycetales
Salinity
Transcriptome
Phosphorylation
Gene Expression
Food

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Medicine(all)

Cite this

Pho85 kinase, a cyclin-dependent kinase, regulates nuclear accumulation of the Rim101 transcription factor in the stress response of Saccharomyces cerevisiae. / Nishizawa, Masafumi; Tanigawa, Mirai; Hayashi, Michio; Maeda, Tatsuya; Yazaki, Yoshiaki; Saeki, Yasushi; Toh-e, Akio.

In: Eukaryotic Cell, Vol. 9, No. 6, 06.2010, p. 943-951.

Research output: Contribution to journalArticle

Nishizawa, Masafumi ; Tanigawa, Mirai ; Hayashi, Michio ; Maeda, Tatsuya ; Yazaki, Yoshiaki ; Saeki, Yasushi ; Toh-e, Akio. / Pho85 kinase, a cyclin-dependent kinase, regulates nuclear accumulation of the Rim101 transcription factor in the stress response of Saccharomyces cerevisiae. In: Eukaryotic Cell. 2010 ; Vol. 9, No. 6. pp. 943-951.
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