Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression of UGP1 encoding UDP-glucose pyrophosphorylase

Masafumi Nishizawa, Maiko Tanabe, Nami Yabuki, Kunio Kitada, Akio Toh-E

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The PHO85 gene is a negative regulator of the PHO system in the yeast Saccharomyces cerevisiae and encodes a protein kinase (Pho85) highly homologous to the Cdc28 kinase (Cdc28). Ten cyclin-like proteins are known to interact with Pho85, and combination with different cyclins is believed to be responsible for distinct Pho85 functions, including phosphate metabolism, carbon source utilization and cell cycle regulation. However, only a limited number of substrates of Pho85 kinase, including Pho4, Gsy2 and Sicl, have so far been identified. To search for more targets of Pho85 and to clarify the genetic control mechanisms by Pho85 kinase in these cellular functions, we carried out a genome-wide analysis of the effect of a pho85Δ mutation on gene expression. We found that expression of various genes involved in carbon metabolism are affected by the mutation and that among them, UGP1 promoter activity was increased in the absence of Pho85 kinase. This increase in the promoter activity was not observed in a pho4Δ mutant or with a mutant UGP1 promoter that is devoid of putative Pho4 and Bas2 binding sites, suggesting that UGP1 expression is modulated by Pho85 through Pho4. We also found that expression of several Pho85-cyclin genes were altered by the carbon source, the growth phase and Pho85 kinase itself.

Original languageEnglish
Pages (from-to)239-249
Number of pages11
JournalYeast
Volume18
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

UTP-Glucose-1-Phosphate Uridylyltransferase
cyclin-dependent kinase
Cyclin-Dependent Kinases
Yeast
Glucose
phosphotransferases (kinases)
Phosphotransferases
Genes
Yeasts
Cyclins
yeasts
cyclins
glucose
Metabolism
Carbon
promoter regions
carbon
Proteins
Binding sites
mutation

Keywords

  • Carbohydrate metabolism
  • Gene expression
  • Glycogen
  • Growth control
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression of UGP1 encoding UDP-glucose pyrophosphorylase. / Nishizawa, Masafumi; Tanabe, Maiko; Yabuki, Nami; Kitada, Kunio; Toh-E, Akio.

In: Yeast, Vol. 18, No. 3, 2001, p. 239-249.

Research output: Contribution to journalArticle

Nishizawa, Masafumi ; Tanabe, Maiko ; Yabuki, Nami ; Kitada, Kunio ; Toh-E, Akio. / Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression of UGP1 encoding UDP-glucose pyrophosphorylase. In: Yeast. 2001 ; Vol. 18, No. 3. pp. 239-249.
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