Phorbol ester enhances choline uptake in cultured porcine brain endothelial cells

Takeshi Suzuki, Chiaki Ikeda, Takeshi Fujii, Kazuko Fujimoto, Ikuo Morita, Sei Itsu Murota, Koichiro Kawashima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Choline uptake in cultured porcine endothelial cells isolated from cerebral microvessels was investigated. A large part of the choline that was taken up was incorporated into phospholipids within 24 h. Phorbol 12-myristate 13-acetate (PMA, 10-7 M), a protein kinase C activator, enhanced choline uptake and acetylcholine (ACh) synthesis in endothelial cells. The PMA-induced enhancement of choline uptake was neither affected by calphostin C (10-7 M), an inhibitor of protein kinase C, nor hemicholinium 3 (10-5 M) treatment. These results suggest that brain endothelial cells have a choline uptake system, which is not identical to the high-affinity choline uptake system located in cholinergic neurons, and that the PMA-induced enhancement of choline uptake is independent of protein kinase C activation. Some of the choline taken up from the extracellular space is used for acetylcholine synthesis.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalBiogenic Amines
Volume12
Issue number1
Publication statusPublished - 1996 Jan 1

Keywords

  • Acetylcholine
  • Choline
  • Endothelial cell
  • Phorbol 12-myristate 13-acetate (PMA)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pharmacology

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  • Cite this

    Suzuki, T., Ikeda, C., Fujii, T., Fujimoto, K., Morita, I., Murota, S. I., & Kawashima, K. (1996). Phorbol ester enhances choline uptake in cultured porcine brain endothelial cells. Biogenic Amines, 12(1), 49-54.