Phorbol myristate acetate stimulates degradation of a structural analogue of platelet-activating factor to a neutral lipid in human leukemic K562 cells: Relevance to the release of lipids

Toshihiko Tsutsumi, Akira Tokumura, Masaya Yamaguchi, Shikifumi Kitazawa, Yusuke Tanigawara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In our attempt to investigate the mechanism of the release of platelet-activating factor (PAF) from cells, the erythroleukemic cell line K562 was preloaded with a radiolabeled PAF analogue having an ethylcarbamyl residue, 1-O-octadecyl-2-O-ethylcarbamyl-sn-glycero-3-phosphocholine (ethylcarbamyl-PAF), that is resistant to the hydrolytic action of PAF acetylhydrolase. Its extracellular release was monitored using an albumin back-extraction method, and its metabolic degradation was analyzed by TLC. Phorbol myristate acetate (PMA) was found to stimulate the release of two radioactive lipids, ethylcarbamyl-PAF itself and its metabolite, 1-O-octadecyl-2-ethylcarbamyl-sn-glycerol, whereas only ethylcarbamyl-PAF was released from the resting cells. The increased release of radioactive lipids in PMA-stimulated cells was suggested to be due to stimulated degradation of intracellular ethylcarbamyl-PAF into the cell-permeable metabolite. Thus K562 cells have much less capacity to release intact PAF-like lipid in comparison with its high ability to uptake exogenously added PAF analogues previously described by us and others.

Original languageEnglish
Pages (from-to)24-28
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number1
DOIs
Publication statusPublished - 2004 Jan 1

Keywords

  • K562 cell
  • Octadecyl-ethylcarbamyl-glycerol
  • PAF analogue
  • Phorbol myristate acetate
  • Platelet-activating factor (PAF)

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Phorbol myristate acetate stimulates degradation of a structural analogue of platelet-activating factor to a neutral lipid in human leukemic K562 cells: Relevance to the release of lipids'. Together they form a unique fingerprint.

  • Cite this