Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice

Kan Handa, Mitsuyo Ohmura, Chiyoko Nishime, Takako Hishiki, Yoshiko Nagahata, Kenji Kawai, Hiroshi Suemizu, Masato Nakamura, Masatoshi Wakui, Yuukou Kitagawa, Makoto Suematsu, Kosuke Tsukada

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

We aimed to examine metabolism of human cancer in vivo and utilized superimmunodeficient NOG mice as an experimental model of hepatic metastasis, where human colon cancer cell line HCT116 transfected with Venus, the mutant GFP was injected intrasplenically. The mice were pretreated with Pd-porphyrin to quantify local O2 tension through intravital phosphorescence assay. In this model, a majority of metastatic foci occurred in periportal regions but not in central regions. At 1 week after the transplantation, a PO2 drop in periportal regions was minimal without any notable decrease in microvascular blood flow. Under these conditions, there was a negative correlation between the size of metastatic foci and the lobular O2 consumption, suggesting that the tumor O2 consumption is smaller than that in the residual liver. At 2 weeks, portal PO2 was significantly smaller than controls, while the central PO2 was not comparably decreased, indicating that metastatic foci increased the O2 consumption, while the residual liver decreased it. These results suggest metastatic tumors derived from human colon cancer exhibit notable aerobic metabolism during their developmental process, compromising respiration of the rest of the tissue regenerated during tumor development.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages423-429
Number of pages7
Volume662
DOIs
Publication statusPublished - 2010

Publication series

NameAdvances in Experimental Medicine and Biology
Volume662
ISSN (Print)00652598

Fingerprint

Phosphorescence
Liver Neoplasms
Liver
Colonic Neoplasms
Tumors
Oxygen
Metabolism
Neoplasms
Venus
Assays
Blood
Cells
Tissue
Respiration
Theoretical Models
Transplantation
Neoplasm Metastasis
Cell Line

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Handa, K., Ohmura, M., Nishime, C., Hishiki, T., Nagahata, Y., Kawai, K., ... Tsukada, K. (2010). Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. In Advances in Experimental Medicine and Biology (Vol. 662, pp. 423-429). (Advances in Experimental Medicine and Biology; Vol. 662). https://doi.org/10.1007/978-1-4419-1241-1_61

Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. / Handa, Kan; Ohmura, Mitsuyo; Nishime, Chiyoko; Hishiki, Takako; Nagahata, Yoshiko; Kawai, Kenji; Suemizu, Hiroshi; Nakamura, Masato; Wakui, Masatoshi; Kitagawa, Yuukou; Suematsu, Makoto; Tsukada, Kosuke.

Advances in Experimental Medicine and Biology. Vol. 662 2010. p. 423-429 (Advances in Experimental Medicine and Biology; Vol. 662).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Handa, K, Ohmura, M, Nishime, C, Hishiki, T, Nagahata, Y, Kawai, K, Suemizu, H, Nakamura, M, Wakui, M, Kitagawa, Y, Suematsu, M & Tsukada, K 2010, Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. in Advances in Experimental Medicine and Biology. vol. 662, Advances in Experimental Medicine and Biology, vol. 662, pp. 423-429. https://doi.org/10.1007/978-1-4419-1241-1_61
Handa K, Ohmura M, Nishime C, Hishiki T, Nagahata Y, Kawai K et al. Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. In Advances in Experimental Medicine and Biology. Vol. 662. 2010. p. 423-429. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4419-1241-1_61
Handa, Kan ; Ohmura, Mitsuyo ; Nishime, Chiyoko ; Hishiki, Takako ; Nagahata, Yoshiko ; Kawai, Kenji ; Suemizu, Hiroshi ; Nakamura, Masato ; Wakui, Masatoshi ; Kitagawa, Yuukou ; Suematsu, Makoto ; Tsukada, Kosuke. / Phosphorescence-assisted microvascular O2 measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice. Advances in Experimental Medicine and Biology. Vol. 662 2010. pp. 423-429 (Advances in Experimental Medicine and Biology).
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