Phosphorylation of RNA polymerase II in cardiac hypertrophy: Cell enlargement signals converge on cyclin T/Cdk9

Prathit A. Kulkarni, Motoaki Sano, Michael D. Schneider

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cardiac myocyte enlargement is the eponymous characteristic of cardiac hypertrophy, regardless of the instigating signal. Such triggers include biomechanical stress (e.g., work load, compensation for ischemic damage), sarcomeric protein mutations, cytoskeletal protein mutations, abnormal energetics, G protein-coupled receptors for ligands (including angiotensin II and endothelin-1), or their signal transducers within cells. In turn, increased myocyte size reflects increased RNA and protein content per cell as responses to these stimuli. In eukaryotic cells, the large subunit of RNA polymerase II (RNAPII) becomes extensively phosphorylated in its serine-rich C-terminal domain (CTD) during the transition from transcript initiation to transcript elongation -that is, "escape" of RNAPII from the promoter-proximal region into the open reading frame. Although this process is believed to be crucial to productive synthesis of mRNA and is known to be governed by two atypical cyclin-dependent kinases, Cdk7 and Cdk9, surprisingly little is understood of how regulatory pathways within cells intersect these RNAPII-directed protein kinases. Investigations of the CTD kinase module in cardiac hypertrophy provide a tentative initial map of a molecular circuit controlling cell size through regulated phosphorylation of RNAPII.

Original languageEnglish
Pages (from-to)125-139
Number of pages15
JournalRecent Progress in Hormone Research
Volume59
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Cyclin T
Cell Enlargement
RNA Polymerase II
Cardiomegaly
Phosphorylation
Mutation
Cytoskeletal Proteins
Cyclin-Dependent Kinases
Eukaryotic Cells
Endothelin-1
G-Protein-Coupled Receptors
Workload
Transducers
Cell Size
Cardiac Myocytes
Genetic Promoter Regions
Angiotensin II
Protein Kinases
Muscle Cells
Serine

ASJC Scopus subject areas

  • Endocrinology

Cite this

Phosphorylation of RNA polymerase II in cardiac hypertrophy : Cell enlargement signals converge on cyclin T/Cdk9. / Kulkarni, Prathit A.; Sano, Motoaki; Schneider, Michael D.

In: Recent Progress in Hormone Research, Vol. 59, 2004, p. 125-139.

Research output: Contribution to journalArticle

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