Phosphorylation of Sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation

Masafumi Nishizawa, Masaoki Kawasumi, Marie Fujino, Akio Toh-e

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

In the yeast Saccharomyces cerevisiae, Sic1, an inhibitor of Clb-Cdc28 kinases, must be phosphorylated and degraded in G1 for cells to initiate DNA replication, and Cln-Cdc28 kinase appears to be primarily responsible for phosphorylation of Sic1. The Pho85 kinase is a yeast cyclin-dependent kinase (Cdk), which is not essential for cell growth unless both CLN1 and CLN2 are absent. We demonstrate that Pho85, when complexed with Pcl1, a G1 cyclin homologue, can phosphorylate Sic1 in vitro, and that Sic1 appears to be more stable in pho85Δ cells. Three consensus Cdk phosphorylation sites present in Sic1 are phosphorylated in vivo, and two of them are required for prompt degradation of the inhibitor. Pho85 and other G1 Cdks appear to phosphorylate Sic1 at different sites in vivo. Thus at least two distinct Cdks can participate in phosphorylation of Sic1 and may therefore regulate progression through G1.

Original languageEnglish
Pages (from-to)2393-2405
Number of pages13
JournalMolecular Biology of the Cell
Volume9
Issue number9
Publication statusPublished - 1998 Sep

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Cyclin-Dependent Kinases
Phosphotransferases
Phosphorylation
Yeasts
Cyclin G1
DNA Replication
Saccharomyces cerevisiae
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Phosphorylation of Sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation. / Nishizawa, Masafumi; Kawasumi, Masaoki; Fujino, Marie; Toh-e, Akio.

In: Molecular Biology of the Cell, Vol. 9, No. 9, 09.1998, p. 2393-2405.

Research output: Contribution to journalArticle

Nishizawa, Masafumi ; Kawasumi, Masaoki ; Fujino, Marie ; Toh-e, Akio. / Phosphorylation of Sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation. In: Molecular Biology of the Cell. 1998 ; Vol. 9, No. 9. pp. 2393-2405.
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