Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics

Naoki Kanoh, Kaori Honda, Siro Simizu, Makoto Muroi, Hiroyuki Osada

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

(Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics.

Original languageEnglish
Pages (from-to)3559-3562
Number of pages4
JournalAngewandte Chemie - International Edition
Volume44
Issue number23
DOIs
Publication statusPublished - 2005 Jun 6
Externally publishedYes

Fingerprint

Molecules
Sepharose
Peptides
Carrier Proteins
Proteins
Genetics

Keywords

  • Affinity purification
  • Immobilization
  • Molecular targeting
  • Peptides
  • Photochemistry

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics. / Kanoh, Naoki; Honda, Kaori; Simizu, Siro; Muroi, Makoto; Osada, Hiroyuki.

In: Angewandte Chemie - International Edition, Vol. 44, No. 23, 06.06.2005, p. 3559-3562.

Research output: Contribution to journalArticle

Kanoh, Naoki ; Honda, Kaori ; Simizu, Siro ; Muroi, Makoto ; Osada, Hiroyuki. / Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics. In: Angewandte Chemie - International Edition. 2005 ; Vol. 44, No. 23. pp. 3559-3562.
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