Photoacoustic imaging for transvascular drug delivery to the rat brain

Ryota Watanabe, Shunichi Sato, Yasuyuki Tsunoi, Satoko Kawauchi, Toshiya Takemura, Mitsuhiro Terakawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Transvascular drug delivery to the brain is difficult due to the blood-brain barrier (BBB). Thus, various methods for safely opening the BBB have been investigated, for which real-time imaging methods are desired both for the blood vessels and distribution of a drug. Photoacoustic (PA) imaging, which enables depth-resolved visualization of chromophores in tissue, would be useful for this purpose. In this study, we performed in vivo PA imaging of the blood vessels and distribution of a drug in the rat brain by using an originally developed compact PA imaging system with fiber-based illumination. As a test drug, Evans blue (EB) was injected to the tail vein, and a photomechanical wave was applied to the targeted brain tissue to increase the permeability of the blood vessel walls. For PA imaging of blood vessels and EB distribution, nanosecond pulses at 532 nm and 670 nm were used, respectively. We clearly visualized blood vessels with diameters larger than 50 μm and the distribution of EB in the brain, showing spatiotemporal characteristics of EB that was transvascularly delivered to the target tissue in the brain.

Original languageEnglish
Title of host publicationOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
EditorsHenry Hirschberg, Steen J. Madsen, Qingming Luo, Samarendra K. Mohanty, Nitish V. Thakor, E. Duco Jansen
PublisherSPIE
ISBN (Electronic)9781628413953
DOIs
Publication statusPublished - 2015 Jan 1
EventOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II - San Francisco, United States
Duration: 2015 Feb 72015 Feb 10

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9305
ISSN (Print)1605-7422

Other

OtherOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
CountryUnited States
CitySan Francisco
Period15/2/715/2/10

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Keywords

  • blood vessels
  • blood-brain barrier
  • drug delivery
  • optical fiber
  • photoacoustic imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Watanabe, R., Sato, S., Tsunoi, Y., Kawauchi, S., Takemura, T., & Terakawa, M. (2015). Photoacoustic imaging for transvascular drug delivery to the rat brain. In H. Hirschberg, S. J. Madsen, Q. Luo, S. K. Mohanty, N. V. Thakor, & E. D. Jansen (Eds.), Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II [930523] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9305). SPIE. https://doi.org/10.1117/12.2078505