Photoacoustic imaging for transvascular drug delivery to the rat brain

Ryota Watanabe, Shunichi Sato, Yasuyuki Tsunoi, Satoko Kawauchi, Toshiya Takemura, Mitsuhiro Terakawa

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)

    Abstract

    Transvascular drug delivery to the brain is difficult due to the blood-brain barrier (BBB). Thus, various methods for safely opening the BBB have been investigated, for which real-time imaging methods are desired both for the blood vessels and distribution of a drug. Photoacoustic (PA) imaging, which enables depth-resolved visualization of chromophores in tissue, would be useful for this purpose. In this study, we performed in vivo PA imaging of the blood vessels and distribution of a drug in the rat brain by using an originally developed compact PA imaging system with fiber-based illumination. As a test drug, Evans blue (EB) was injected to the tail vein, and a photomechanical wave was applied to the targeted brain tissue to increase the permeability of the blood vessel walls. For PA imaging of blood vessels and EB distribution, nanosecond pulses at 532 nm and 670 nm were used, respectively. We clearly visualized blood vessels with diameters larger than 50 μm and the distribution of EB in the brain, showing spatiotemporal characteristics of EB that was transvascularly delivered to the target tissue in the brain.

    Original languageEnglish
    Title of host publicationOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
    EditorsHenry Hirschberg, Steen J. Madsen, Qingming Luo, Samarendra K. Mohanty, Nitish V. Thakor, E. Duco Jansen
    PublisherSPIE
    ISBN (Electronic)9781628413953
    DOIs
    Publication statusPublished - 2015 Jan 1
    EventOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II - San Francisco, United States
    Duration: 2015 Feb 72015 Feb 10

    Publication series

    NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    Volume9305
    ISSN (Print)1605-7422

    Other

    OtherOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
    CountryUnited States
    CitySan Francisco
    Period15/2/715/2/10

    Keywords

    • blood vessels
    • blood-brain barrier
    • drug delivery
    • optical fiber
    • photoacoustic imaging

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Atomic and Molecular Physics, and Optics
    • Radiology Nuclear Medicine and imaging

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  • Cite this

    Watanabe, R., Sato, S., Tsunoi, Y., Kawauchi, S., Takemura, T., & Terakawa, M. (2015). Photoacoustic imaging for transvascular drug delivery to the rat brain. In H. Hirschberg, S. J. Madsen, Q. Luo, S. K. Mohanty, N. V. Thakor, & E. D. Jansen (Eds.), Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II [930523] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9305). SPIE. https://doi.org/10.1117/12.2078505