Photochemically induced increase in endothelial permeablity regulated by RhoA activation

Hiroki Ota, Mimiko Matsumura, Norihisa Miki, Haruyuki Minamitami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Photochemical reactions used in photodynamic therapy are reported to damage normal cells and tissues in ways that increase endothelial permeability and thereby cause excessive neointimal formation and subsequent restenosis. To investigate the mechanisms of this permeability increase in vitro, human umbilical vein endothelial cells were incubated with the porphyrin precursor δ-aminolevulinic acid and then irradiated with a 646 nm light-emitting diode (LED). Results using Transwells® supports showed that the photochemical reaction increased endothelial permeability by 200%, and fluorescence microscopy revealed that destruction of the capillary-like structures due to cell shrinkage was accompanied by VE-cadherin mislocalization and stress fiber formation. The generated gaps between cells were observed using dyed β1-integrin and total internal reflection fluorescence microscopy. Western blotting indicated that the photochemical reaction phosphorylated GDP-RhoA to GTP-RhoA, a protein that promotes stress fiber formation and inhibits VE-cadherin production. When forskolin/rolipram or 8CPT-2′O-Me- cAMP, both of which inhibit further reaction of phosphorylated RhoA, were added, no formation of stress fibers or mislocalization of VE-cadherin was observed, thus preventing an increase in endothelial permeability. Taken together, photochemically induced RhoA activation appears to play a key role in increasing endothelial permeability during changes in morphology of endothelial cells.

Original languageEnglish
Pages (from-to)1401-1407
Number of pages7
JournalPhotochemical and Photobiological Sciences
Volume8
Issue number10
DOIs
Publication statusPublished - 2009

Fingerprint

Photochemical reactions
permeability
Fluorescence microscopy
Chemical activation
Endothelial cells
activation
Fibers
photochemical reactions
Rolipram
Aminolevulinic Acid
Photodynamic therapy
Porphyrins
fibers
Colforsin
Heat-Shock Proteins
Guanosine Triphosphate
Integrins
cells
Light emitting diodes
microscopy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Photochemically induced increase in endothelial permeablity regulated by RhoA activation. / Ota, Hiroki; Matsumura, Mimiko; Miki, Norihisa; Minamitami, Haruyuki.

In: Photochemical and Photobiological Sciences, Vol. 8, No. 10, 2009, p. 1401-1407.

Research output: Contribution to journalArticle

Ota, Hiroki ; Matsumura, Mimiko ; Miki, Norihisa ; Minamitami, Haruyuki. / Photochemically induced increase in endothelial permeablity regulated by RhoA activation. In: Photochemical and Photobiological Sciences. 2009 ; Vol. 8, No. 10. pp. 1401-1407.
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