PhotonSABER

new tool shedding light on endocytosis and learning mechanisms in vivo

Research output: Contribution to journalArticle

Abstract

In the central nervous system, activity-dependent endocytosis of postsynaptic AMPA-type glutamate receptors (AMPA receptors) is thought to mediate long-term depression (LTD), which is a synaptic plasticity model in various neuronal circuits. However, whether and how AMPA receptor endocytosis and LTD at specific synapses are causally linked to learning and memory in vivo remains unclear. Recently, we developed a new optogenetic tool, PhotonSABER, which could control AMPA receptor endocytosis in temporal, spatial, and cell-type-specific manners at activated synapses. Using PhotonSABER, we found that AMPA receptor endocytosis and LTD at synapses between parallel fibers and Purkinje cells in the cerebellum mediate oculomotor learning. We also found that PhotonSABER could inhibit endocytosis of epidermal growth factor receptors in HeLa cells upon light stimulation. These results demonstrate that PhotonSABER is a powerful tool for analyzing the physiological functions of endocytosis in non-neuronal cells, as well as the roles of LTD in various brain regions.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalCommunicative and Integrative Biology
Volume12
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

endocytosis
Endocytosis
AMPA Receptors
learning
Learning
synapse
Synapses
Depression
receptors
Optogenetics
cells
Neuronal Plasticity
Purkinje Cells
Glutamate Receptors
cerebellum
HeLa Cells
Cerebellum
central nervous system
Central Nervous System
brain

Keywords

  • AMPA receptor
  • Long-term depression
  • optogenetics
  • Purkinje cell
  • synapse

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

PhotonSABER : new tool shedding light on endocytosis and learning mechanisms in vivo. / Matsuda, Shinji; Kakegawa, Wataru; Yuzaki, Michisuke.

In: Communicative and Integrative Biology, Vol. 12, No. 1, 01.01.2019, p. 34-37.

Research output: Contribution to journalArticle

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