Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution

A. Ito, H. Matsuo, T. Suenari, T. Kimura, S. Miyoshi, S. Ogawa, T. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have demonstrated photosensitization reaction-induced electrical conduction block of myocardial tissue. Subcellular distribution of photosensitizer and electrophysiological cell response to this photosensitization reaction were studied with rat myocardial cells, in order to clarify the mechanism of the myocardial electrical conduction block with photodynamic therapy (PDT). Photofrin® was used as photo sensitizer in this study. The subcellular distribution of the photosensitizer was obtained by microscopic fluorescent observation. The sites of photosensitizer localization were deter mined by organelle fluorescent probe, Rhodamine® 123, to specify the mitochondria location. The myocardial cell response to the photosensitization reaction was studied by the observation of intracellular Ca2+ dynamics. Intracellular free Ca2+concentration in the myocardial cells was measured with fluorescent Ca2+ indicator, Fluo-4 AM, by a high-speed Nipkow type confocal laser microscope during the PDT when the photosensitizer was distributed outside the cells or localized to intracellular compartments. We concluded that the Ca2+ influx accompanied by cell membrane damage when the photosensitizer was distributed outside the cells or on the cell membrane was the possible mechanism of the myocardial electrical conduction block with the photosensitization reaction.

Original languageEnglish
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Subtitle of host publicationDiagnostic and Therapeutic Instrumentation, Clinical Engineering
Pages229-231
Number of pages3
Edition7
DOIs
Publication statusPublished - 2009 Dec 1
EventWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering - Munich, Germany
Duration: 2009 Sep 72009 Sep 12

Publication series

NameIFMBE Proceedings
Number7
Volume25
ISSN (Print)1680-0737

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering
CountryGermany
CityMunich
Period09/9/709/9/12

Keywords

  • Ca
  • Electrical conduction block
  • Photodynamic therapy
  • Photofrin®
  • Photosensitization reaction

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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    Ito, A., Matsuo, H., Suenari, T., Kimura, T., Miyoshi, S., Ogawa, S., & Arai, T. (2009). Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution. In World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering (7 ed., pp. 229-231). (IFMBE Proceedings; Vol. 25, No. 7). https://doi.org/10.1007/978-3-642-03885-3-64