Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution

A. Ito, H. Matsuo, T. Suenari, T. Kimura, S. Miyoshi, S. Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have demonstrated photosensitization reaction-induced electrical conduction block of myocardial tissue. Subcellular distribution of photosensitizer and electrophysiological cell response to this photosensitization reaction were studied with rat myocardial cells, in order to clarify the mechanism of the myocardial electrical conduction block with photodynamic therapy (PDT). Photofrin® was used as photo sensitizer in this study. The subcellular distribution of the photosensitizer was obtained by microscopic fluorescent observation. The sites of photosensitizer localization were deter mined by organelle fluorescent probe, Rhodamine® 123, to specify the mitochondria location. The myocardial cell response to the photosensitization reaction was studied by the observation of intracellular Ca2+ dynamics. Intracellular free Ca2+concentration in the myocardial cells was measured with fluorescent Ca2+ indicator, Fluo-4 AM, by a high-speed Nipkow type confocal laser microscope during the PDT when the photosensitizer was distributed outside the cells or localized to intracellular compartments. We concluded that the Ca2+ influx accompanied by cell membrane damage when the photosensitizer was distributed outside the cells or on the cell membrane was the possible mechanism of the myocardial electrical conduction block with the photosensitization reaction.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages229-231
Number of pages3
Volume25
Edition7
DOIs
Publication statusPublished - 2009
EventWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering - Munich, Germany
Duration: 2009 Sep 72009 Sep 12

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering
CountryGermany
CityMunich
Period09/9/709/9/12

Fingerprint

Photosensitizing Agents
Photosensitizers
Photodynamic therapy
Cell membranes
Dihematoporphyrin Ether
Rhodamine 123
Mitochondria
Fluorescent Dyes
Rats
Microscopes
Tissue
Lasers

Keywords

  • Ca
  • Electrical conduction block
  • Photodynamic therapy
  • Photofrin®
  • Photosensitization reaction

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Ito, A., Matsuo, H., Suenari, T., Kimura, T., Miyoshi, S., Ogawa, S., & Arai, T. (2009). Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution. In IFMBE Proceedings (7 ed., Vol. 25, pp. 229-231) https://doi.org/10.1007/978-3-642-03885-3-64

Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution. / Ito, A.; Matsuo, H.; Suenari, T.; Kimura, T.; Miyoshi, S.; Ogawa, S.; Arai, Tsunenori.

IFMBE Proceedings. Vol. 25 7. ed. 2009. p. 229-231.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ito, A, Matsuo, H, Suenari, T, Kimura, T, Miyoshi, S, Ogawa, S & Arai, T 2009, Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution. in IFMBE Proceedings. 7 edn, vol. 25, pp. 229-231, World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering, Munich, Germany, 09/9/7. https://doi.org/10.1007/978-3-642-03885-3-64
Ito, A. ; Matsuo, H. ; Suenari, T. ; Kimura, T. ; Miyoshi, S. ; Ogawa, S. ; Arai, Tsunenori. / Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution. IFMBE Proceedings. Vol. 25 7. ed. 2009. pp. 229-231
@inproceedings{a71b308eae2a405280670c366d6ddb61,
title = "Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution",
abstract = "We have demonstrated photosensitization reaction-induced electrical conduction block of myocardial tissue. Subcellular distribution of photosensitizer and electrophysiological cell response to this photosensitization reaction were studied with rat myocardial cells, in order to clarify the mechanism of the myocardial electrical conduction block with photodynamic therapy (PDT). Photofrin{\circledR} was used as photo sensitizer in this study. The subcellular distribution of the photosensitizer was obtained by microscopic fluorescent observation. The sites of photosensitizer localization were deter mined by organelle fluorescent probe, Rhodamine{\circledR} 123, to specify the mitochondria location. The myocardial cell response to the photosensitization reaction was studied by the observation of intracellular Ca2+ dynamics. Intracellular free Ca2+concentration in the myocardial cells was measured with fluorescent Ca2+ indicator, Fluo-4 AM, by a high-speed Nipkow type confocal laser microscope during the PDT when the photosensitizer was distributed outside the cells or localized to intracellular compartments. We concluded that the Ca2+ influx accompanied by cell membrane damage when the photosensitizer was distributed outside the cells or on the cell membrane was the possible mechanism of the myocardial electrical conduction block with the photosensitization reaction.",
keywords = "Ca, Electrical conduction block, Photodynamic therapy, Photofrin{\circledR}, Photosensitization reaction",
author = "A. Ito and H. Matsuo and T. Suenari and T. Kimura and S. Miyoshi and S. Ogawa and Tsunenori Arai",
year = "2009",
doi = "10.1007/978-3-642-03885-3-64",
language = "English",
isbn = "9783642038846",
volume = "25",
pages = "229--231",
booktitle = "IFMBE Proceedings",
edition = "7",

}

TY - GEN

T1 - Photosensitization reaction-induced electrophysiological response of myocardial cells dependent on subcellular photosensitizer distribution

AU - Ito, A.

AU - Matsuo, H.

AU - Suenari, T.

AU - Kimura, T.

AU - Miyoshi, S.

AU - Ogawa, S.

AU - Arai, Tsunenori

PY - 2009

Y1 - 2009

N2 - We have demonstrated photosensitization reaction-induced electrical conduction block of myocardial tissue. Subcellular distribution of photosensitizer and electrophysiological cell response to this photosensitization reaction were studied with rat myocardial cells, in order to clarify the mechanism of the myocardial electrical conduction block with photodynamic therapy (PDT). Photofrin® was used as photo sensitizer in this study. The subcellular distribution of the photosensitizer was obtained by microscopic fluorescent observation. The sites of photosensitizer localization were deter mined by organelle fluorescent probe, Rhodamine® 123, to specify the mitochondria location. The myocardial cell response to the photosensitization reaction was studied by the observation of intracellular Ca2+ dynamics. Intracellular free Ca2+concentration in the myocardial cells was measured with fluorescent Ca2+ indicator, Fluo-4 AM, by a high-speed Nipkow type confocal laser microscope during the PDT when the photosensitizer was distributed outside the cells or localized to intracellular compartments. We concluded that the Ca2+ influx accompanied by cell membrane damage when the photosensitizer was distributed outside the cells or on the cell membrane was the possible mechanism of the myocardial electrical conduction block with the photosensitization reaction.

AB - We have demonstrated photosensitization reaction-induced electrical conduction block of myocardial tissue. Subcellular distribution of photosensitizer and electrophysiological cell response to this photosensitization reaction were studied with rat myocardial cells, in order to clarify the mechanism of the myocardial electrical conduction block with photodynamic therapy (PDT). Photofrin® was used as photo sensitizer in this study. The subcellular distribution of the photosensitizer was obtained by microscopic fluorescent observation. The sites of photosensitizer localization were deter mined by organelle fluorescent probe, Rhodamine® 123, to specify the mitochondria location. The myocardial cell response to the photosensitization reaction was studied by the observation of intracellular Ca2+ dynamics. Intracellular free Ca2+concentration in the myocardial cells was measured with fluorescent Ca2+ indicator, Fluo-4 AM, by a high-speed Nipkow type confocal laser microscope during the PDT when the photosensitizer was distributed outside the cells or localized to intracellular compartments. We concluded that the Ca2+ influx accompanied by cell membrane damage when the photosensitizer was distributed outside the cells or on the cell membrane was the possible mechanism of the myocardial electrical conduction block with the photosensitization reaction.

KW - Ca

KW - Electrical conduction block

KW - Photodynamic therapy

KW - Photofrin®

KW - Photosensitization reaction

UR - http://www.scopus.com/inward/record.url?scp=77949785722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949785722&partnerID=8YFLogxK

U2 - 10.1007/978-3-642-03885-3-64

DO - 10.1007/978-3-642-03885-3-64

M3 - Conference contribution

AN - SCOPUS:77949785722

SN - 9783642038846

VL - 25

SP - 229

EP - 231

BT - IFMBE Proceedings

ER -