Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating

Makoto Ogura, Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Minoru Obara

    Research output: Contribution to journalConference article

    2 Citations (Scopus)

    Abstract

    In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofim) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 °C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4′, 6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95 %). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.

    Original languageEnglish
    Pages (from-to)10-12
    Number of pages3
    JournalProceedings of SPIE - The International Society for Optical Engineering
    Volume4949
    DOIs
    Publication statusPublished - 2003 Sep 29
    EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII - San Jose,CA, United States
    Duration: 2003 Jan 252003 Jan 26

    Keywords

    • Drug delivery
    • Fluidity
    • Heating
    • Laser-induced stress wave
    • Lipid bilayer
    • Photofrin
    • Photosensitizer
    • Plasma membrane
    • Porfimer sodium
    • Skin

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics
    • Computer Science Applications
    • Applied Mathematics
    • Electrical and Electronic Engineering

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