Abstract
In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofim) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 °C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4′, 6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95 %). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | L.S. Bass, N Kollias, R.S. Malek, A Katzir, U.K. Shah, B.J.F. Wong, E.A. Trowers, T.A. Woodward, W.T.W. de Riese, D.S. Robinson, H-D Reidenbach, K.D. Paulsen, K.W. Gregory |
| Pages | 10-12 |
| Number of pages | 3 |
| Volume | 4949 |
| DOIs | |
| Publication status | Published - 2003 |
| Event | Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII - San Jose,CA, United States Duration: 2003 Jan 25 → 2003 Jan 26 |
Other
| Other | Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII |
|---|---|
| Country | United States |
| City | San Jose,CA |
| Period | 03/1/25 → 03/1/26 |
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Keywords
- Drug delivery
- Fluidity
- Heating
- Laser-induced stress wave
- Lipid bilayer
- Photofrin
- Photosensitizer
- Plasma membrane
- Porfimer sodium
- Skin
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating. / Ogura, Makoto; Terakawa, Mitsuhiro; Sato, Shunichi; Ashida, Hiroshi; Obara, Minoru.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / L.S. Bass; N Kollias; R.S. Malek; A Katzir; U.K. Shah; B.J.F. Wong; E.A. Trowers; T.A. Woodward; W.T.W. de Riese; D.S. Robinson; H-D Reidenbach; K.D. Paulsen; K.W. Gregory. Vol. 4949 2003. p. 10-12.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating
AU - Ogura, Makoto
AU - Terakawa, Mitsuhiro
AU - Sato, Shunichi
AU - Ashida, Hiroshi
AU - Obara, Minoru
PY - 2003
Y1 - 2003
N2 - In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofim) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 °C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4′, 6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95 %). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.
AB - In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofim) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 °C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4′, 6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95 %). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.
KW - Drug delivery
KW - Fluidity
KW - Heating
KW - Laser-induced stress wave
KW - Lipid bilayer
KW - Photofrin
KW - Photosensitizer
KW - Plasma membrane
KW - Porfimer sodium
KW - Skin
UR - http://www.scopus.com/inward/record.url?scp=0141717879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141717879&partnerID=8YFLogxK
U2 - 10.1117/12.476137
DO - 10.1117/12.476137
M3 - Conference contribution
AN - SCOPUS:0141717879
VL - 4949
SP - 10
EP - 12
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Bass, L.S.
A2 - Kollias, N
A2 - Malek, R.S.
A2 - Katzir, A
A2 - Shah, U.K.
A2 - Wong, B.J.F.
A2 - Trowers, E.A.
A2 - Woodward, T.A.
A2 - de Riese, W.T.W.
A2 - Robinson, D.S.
A2 - Reidenbach, H-D
A2 - Paulsen, K.D.
A2 - Gregory, K.W.
ER -