Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating

Makoto Ogura, Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Minoru Obara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofim) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 °C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4′, 6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95 %). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsL.S. Bass, N Kollias, R.S. Malek, A Katzir, U.K. Shah, B.J.F. Wong, E.A. Trowers, T.A. Woodward, W.T.W. de Riese, D.S. Robinson, H-D Reidenbach, K.D. Paulsen, K.W. Gregory
Pages10-12
Number of pages3
Volume4949
DOIs
Publication statusPublished - 2003
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII - San Jose,CA, United States
Duration: 2003 Jan 252003 Jan 26

Other

OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII
CountryUnited States
CitySan Jose,CA
Period03/1/2503/1/26

Fingerprint

stress waves
Photosensitizers
delivery
Tissue
Skin
Heating
heating
Lasers
cells
lasers
Fibroblasts
fibroblasts
Irradiation
Laser heating
Photodynamic therapy
culture media
irradiation
Cell culture
laser heating
Optical fibers

Keywords

  • Drug delivery
  • Fluidity
  • Heating
  • Laser-induced stress wave
  • Lipid bilayer
  • Photofrin
  • Photosensitizer
  • Plasma membrane
  • Porfimer sodium
  • Skin

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Ogura, M., Terakawa, M., Sato, S., Ashida, H., & Obara, M. (2003). Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating. In L. S. Bass, N. Kollias, R. S. Malek, A. Katzir, U. K. Shah, B. J. F. Wong, E. A. Trowers, T. A. Woodward, W. T. W. de Riese, D. S. Robinson, H-D. Reidenbach, K. D. Paulsen, ... K. W. Gregory (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4949, pp. 10-12) https://doi.org/10.1117/12.476137

Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating. / Ogura, Makoto; Terakawa, Mitsuhiro; Sato, Shunichi; Ashida, Hiroshi; Obara, Minoru.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / L.S. Bass; N Kollias; R.S. Malek; A Katzir; U.K. Shah; B.J.F. Wong; E.A. Trowers; T.A. Woodward; W.T.W. de Riese; D.S. Robinson; H-D Reidenbach; K.D. Paulsen; K.W. Gregory. Vol. 4949 2003. p. 10-12.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogura, M, Terakawa, M, Sato, S, Ashida, H & Obara, M 2003, Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating. in LS Bass, N Kollias, RS Malek, A Katzir, UK Shah, BJF Wong, EA Trowers, TA Woodward, WTW de Riese, DS Robinson, H-D Reidenbach, KD Paulsen & KW Gregory (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4949, pp. 10-12, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, San Jose,CA, United States, 03/1/25. https://doi.org/10.1117/12.476137
Ogura M, Terakawa M, Sato S, Ashida H, Obara M. Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating. In Bass LS, Kollias N, Malek RS, Katzir A, Shah UK, Wong BJF, Trowers EA, Woodward TA, de Riese WTW, Robinson DS, Reidenbach H-D, Paulsen KD, Gregory KW, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4949. 2003. p. 10-12 https://doi.org/10.1117/12.476137
Ogura, Makoto ; Terakawa, Mitsuhiro ; Sato, Shunichi ; Ashida, Hiroshi ; Obara, Minoru. / Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating. Proceedings of SPIE - The International Society for Optical Engineering. editor / L.S. Bass ; N Kollias ; R.S. Malek ; A Katzir ; U.K. Shah ; B.J.F. Wong ; E.A. Trowers ; T.A. Woodward ; W.T.W. de Riese ; D.S. Robinson ; H-D Reidenbach ; K.D. Paulsen ; K.W. Gregory. Vol. 4949 2003. pp. 10-12
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