Abstract
In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofim) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 °C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4′, 6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95 %). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.
Original language | English |
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Pages (from-to) | 10-12 |
Number of pages | 3 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 4949 |
DOIs | |
Publication status | Published - 2003 Sep 29 |
Event | Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII - San Jose,CA, United States Duration: 2003 Jan 25 → 2003 Jan 26 |
Keywords
- Drug delivery
- Fluidity
- Heating
- Laser-induced stress wave
- Lipid bilayer
- Photofrin
- Photosensitizer
- Plasma membrane
- Porfimer sodium
- Skin
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering