Photosensitizer delivery into tissue and cells by simultaneous application of laser-induced stress wave and heating

Makoto Ogura, Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Minoru Obara

Research output: Contribution to journalConference article

2 Citations (Scopus)

Abstract

In order to enhance the efficacy of photodynamic therapy for skin diseases, we previously demonstrated that the simultaneous application of heating and a laser-induced stress wave (LISW) accelerated skin permeabilization of porfimer sodium (Photofim) in in-vivo experiments. In this study, to investigate the uptake of Photofrin by cells within skin, we carried out in-vitro experiments using fibroblast-like cells. Immediately after replacing culture medium with 43 °C phosphate buffered saline solution of Photofrin in a well, the cells were exposed to a LISW that was generated by irradiation of a nanosecond laser pulse onto a target attached on the back of the well. Uptake of Photofrin within the cells was observed with a confocal laser scanning microscope, while the cells were stained with DAPI (4′, 6-diamidino-2-phenylindole) to evaluate the survival rate. The largest uptake was observed for the simultaneous application (heating + LISW) with high survival rates (more than 95 %). These results suggested that Photofrin was transported into fibroblasts within skin. In this method, irradiation of low-fluence laser was effective to enhance uptake, and therefore optical fiber can be used for laser delivery.

Original languageEnglish
Pages (from-to)10-12
Number of pages3
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4949
DOIs
Publication statusPublished - 2003 Sep 29
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII - San Jose,CA, United States
Duration: 2003 Jan 252003 Jan 26

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Keywords

  • Drug delivery
  • Fluidity
  • Heating
  • Laser-induced stress wave
  • Lipid bilayer
  • Photofrin
  • Photosensitizer
  • Plasma membrane
  • Porfimer sodium
  • Skin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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