Photosynthetic production of enantioselective biocatalysts

Maik Bartsch, Sarah K. Gassmeyer, Katharina Köninger, Kosuke Igarashi, Pasqual Liauw, Nina Dyczmons-Nowaczyk, Kenji Miyamoto, Marc M. Nowaczyk, Robert Kourist

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Global resource depletion poses a dramatic threat to our society and creates a strong demand for alternative resources that do not compete with the production of food. Meeting this challenge requires a thorough rethinking of all steps of the value chain regarding their sustainability resource demand and the possibility to substitute current, petrol-based supply-chains with renewable resources. This regards also the production of catalysts for chemical synthesis. Phototrophic microorganisms have attracted considerable attention as a biomanufacturing platform for the sustainable production of chemicals and biofuels. They allow the direct utilization of carbon dioxide and do not compete with food production. Photosynthetic enzyme production of catalysts would be a sustainable supply of these important components of the biotechnological and chemical industries. This paper focuses on the usefulness of recombinant cyanobacteria for the photosynthetic expression of enantioselective catalysts. As a proof of concept, we used the cyanobacterium Synechocystis sp. PCC 6803 for the heterologous expression of two highly enantioselective enzymes. Results: We investigated the expression yield and the usefulness of cyanobacterial cell extracts for conducting stereoselective reactions. The cyanobacterial enzyme expression achieved protein yields of 3% of total soluble protein (%TSP) while the expression in E. coli yielded 6-8% TSP. Cell-free extracts from a recombinant strain expressing the recombinant esterase ST0071 from the thermophilic organism Sulfolobus tokodai ST0071 and arylmalonate decarboxylase from Bordetella bronchiseptica showed excellent enantioselectivity (>99% ee) and yield (>91%) in the desymmetrisation of prochiral malonates. Conclusions: We were able to present the proof-of-concept of photoautotrophic enzyme expression as a viable alternative to heterotrophic expression hosts. Our results show that the introduction of foreign genes is straightforward. Cell components from Synechocystis did not interfere with the stereoselective transformations, underlining the usability of photoautotrophic organisms for the production of enzymes. Given the considerable commercial value of recombinant biocatalysts, cyanobacterial enzyme expression has thus the potential to complement existing approaches to use phototrophic organisms for the production of chemicals and biofuels.

Original languageEnglish
Article number53
JournalMicrobial Cell Factories
Volume14
Issue number1
DOIs
Publication statusPublished - 2015 Apr 15

Fingerprint

Biocatalysts
Enzymes
Synechocystis
Biofuels
Cyanobacteria
Cell Extracts
Catalysts
Malonates
Sulfolobus
Bordetella bronchiseptica
Proteins
Chemical Industry
Food
Enantioselectivity
Esterases
Cellular Structures
Chemical industry
Carbon Dioxide
Microorganisms
Escherichia coli

Keywords

  • Biocatalysis
  • Enantioselectivity
  • Enzyme expression
  • Photosynthesis
  • Sustainability

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Bartsch, M., Gassmeyer, S. K., Köninger, K., Igarashi, K., Liauw, P., Dyczmons-Nowaczyk, N., ... Kourist, R. (2015). Photosynthetic production of enantioselective biocatalysts. Microbial Cell Factories, 14(1), [53]. https://doi.org/10.1186/s12934-015-0233-5

Photosynthetic production of enantioselective biocatalysts. / Bartsch, Maik; Gassmeyer, Sarah K.; Köninger, Katharina; Igarashi, Kosuke; Liauw, Pasqual; Dyczmons-Nowaczyk, Nina; Miyamoto, Kenji; Nowaczyk, Marc M.; Kourist, Robert.

In: Microbial Cell Factories, Vol. 14, No. 1, 53, 15.04.2015.

Research output: Contribution to journalArticle

Bartsch, M, Gassmeyer, SK, Köninger, K, Igarashi, K, Liauw, P, Dyczmons-Nowaczyk, N, Miyamoto, K, Nowaczyk, MM & Kourist, R 2015, 'Photosynthetic production of enantioselective biocatalysts', Microbial Cell Factories, vol. 14, no. 1, 53. https://doi.org/10.1186/s12934-015-0233-5
Bartsch M, Gassmeyer SK, Köninger K, Igarashi K, Liauw P, Dyczmons-Nowaczyk N et al. Photosynthetic production of enantioselective biocatalysts. Microbial Cell Factories. 2015 Apr 15;14(1). 53. https://doi.org/10.1186/s12934-015-0233-5
Bartsch, Maik ; Gassmeyer, Sarah K. ; Köninger, Katharina ; Igarashi, Kosuke ; Liauw, Pasqual ; Dyczmons-Nowaczyk, Nina ; Miyamoto, Kenji ; Nowaczyk, Marc M. ; Kourist, Robert. / Photosynthetic production of enantioselective biocatalysts. In: Microbial Cell Factories. 2015 ; Vol. 14, No. 1.
@article{0857e2d9625d46f2ac929da3e6bb3924,
title = "Photosynthetic production of enantioselective biocatalysts",
abstract = "Background: Global resource depletion poses a dramatic threat to our society and creates a strong demand for alternative resources that do not compete with the production of food. Meeting this challenge requires a thorough rethinking of all steps of the value chain regarding their sustainability resource demand and the possibility to substitute current, petrol-based supply-chains with renewable resources. This regards also the production of catalysts for chemical synthesis. Phototrophic microorganisms have attracted considerable attention as a biomanufacturing platform for the sustainable production of chemicals and biofuels. They allow the direct utilization of carbon dioxide and do not compete with food production. Photosynthetic enzyme production of catalysts would be a sustainable supply of these important components of the biotechnological and chemical industries. This paper focuses on the usefulness of recombinant cyanobacteria for the photosynthetic expression of enantioselective catalysts. As a proof of concept, we used the cyanobacterium Synechocystis sp. PCC 6803 for the heterologous expression of two highly enantioselective enzymes. Results: We investigated the expression yield and the usefulness of cyanobacterial cell extracts for conducting stereoselective reactions. The cyanobacterial enzyme expression achieved protein yields of 3{\%} of total soluble protein ({\%}TSP) while the expression in E. coli yielded 6-8{\%} TSP. Cell-free extracts from a recombinant strain expressing the recombinant esterase ST0071 from the thermophilic organism Sulfolobus tokodai ST0071 and arylmalonate decarboxylase from Bordetella bronchiseptica showed excellent enantioselectivity (>99{\%} ee) and yield (>91{\%}) in the desymmetrisation of prochiral malonates. Conclusions: We were able to present the proof-of-concept of photoautotrophic enzyme expression as a viable alternative to heterotrophic expression hosts. Our results show that the introduction of foreign genes is straightforward. Cell components from Synechocystis did not interfere with the stereoselective transformations, underlining the usability of photoautotrophic organisms for the production of enzymes. Given the considerable commercial value of recombinant biocatalysts, cyanobacterial enzyme expression has thus the potential to complement existing approaches to use phototrophic organisms for the production of chemicals and biofuels.",
keywords = "Biocatalysis, Enantioselectivity, Enzyme expression, Photosynthesis, Sustainability",
author = "Maik Bartsch and Gassmeyer, {Sarah K.} and Katharina K{\"o}ninger and Kosuke Igarashi and Pasqual Liauw and Nina Dyczmons-Nowaczyk and Kenji Miyamoto and Nowaczyk, {Marc M.} and Robert Kourist",
year = "2015",
month = "4",
day = "15",
doi = "10.1186/s12934-015-0233-5",
language = "English",
volume = "14",
journal = "Microbial Cell Factories",
issn = "1475-2859",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Photosynthetic production of enantioselective biocatalysts

AU - Bartsch, Maik

AU - Gassmeyer, Sarah K.

AU - Köninger, Katharina

AU - Igarashi, Kosuke

AU - Liauw, Pasqual

AU - Dyczmons-Nowaczyk, Nina

AU - Miyamoto, Kenji

AU - Nowaczyk, Marc M.

AU - Kourist, Robert

PY - 2015/4/15

Y1 - 2015/4/15

N2 - Background: Global resource depletion poses a dramatic threat to our society and creates a strong demand for alternative resources that do not compete with the production of food. Meeting this challenge requires a thorough rethinking of all steps of the value chain regarding their sustainability resource demand and the possibility to substitute current, petrol-based supply-chains with renewable resources. This regards also the production of catalysts for chemical synthesis. Phototrophic microorganisms have attracted considerable attention as a biomanufacturing platform for the sustainable production of chemicals and biofuels. They allow the direct utilization of carbon dioxide and do not compete with food production. Photosynthetic enzyme production of catalysts would be a sustainable supply of these important components of the biotechnological and chemical industries. This paper focuses on the usefulness of recombinant cyanobacteria for the photosynthetic expression of enantioselective catalysts. As a proof of concept, we used the cyanobacterium Synechocystis sp. PCC 6803 for the heterologous expression of two highly enantioselective enzymes. Results: We investigated the expression yield and the usefulness of cyanobacterial cell extracts for conducting stereoselective reactions. The cyanobacterial enzyme expression achieved protein yields of 3% of total soluble protein (%TSP) while the expression in E. coli yielded 6-8% TSP. Cell-free extracts from a recombinant strain expressing the recombinant esterase ST0071 from the thermophilic organism Sulfolobus tokodai ST0071 and arylmalonate decarboxylase from Bordetella bronchiseptica showed excellent enantioselectivity (>99% ee) and yield (>91%) in the desymmetrisation of prochiral malonates. Conclusions: We were able to present the proof-of-concept of photoautotrophic enzyme expression as a viable alternative to heterotrophic expression hosts. Our results show that the introduction of foreign genes is straightforward. Cell components from Synechocystis did not interfere with the stereoselective transformations, underlining the usability of photoautotrophic organisms for the production of enzymes. Given the considerable commercial value of recombinant biocatalysts, cyanobacterial enzyme expression has thus the potential to complement existing approaches to use phototrophic organisms for the production of chemicals and biofuels.

AB - Background: Global resource depletion poses a dramatic threat to our society and creates a strong demand for alternative resources that do not compete with the production of food. Meeting this challenge requires a thorough rethinking of all steps of the value chain regarding their sustainability resource demand and the possibility to substitute current, petrol-based supply-chains with renewable resources. This regards also the production of catalysts for chemical synthesis. Phototrophic microorganisms have attracted considerable attention as a biomanufacturing platform for the sustainable production of chemicals and biofuels. They allow the direct utilization of carbon dioxide and do not compete with food production. Photosynthetic enzyme production of catalysts would be a sustainable supply of these important components of the biotechnological and chemical industries. This paper focuses on the usefulness of recombinant cyanobacteria for the photosynthetic expression of enantioselective catalysts. As a proof of concept, we used the cyanobacterium Synechocystis sp. PCC 6803 for the heterologous expression of two highly enantioselective enzymes. Results: We investigated the expression yield and the usefulness of cyanobacterial cell extracts for conducting stereoselective reactions. The cyanobacterial enzyme expression achieved protein yields of 3% of total soluble protein (%TSP) while the expression in E. coli yielded 6-8% TSP. Cell-free extracts from a recombinant strain expressing the recombinant esterase ST0071 from the thermophilic organism Sulfolobus tokodai ST0071 and arylmalonate decarboxylase from Bordetella bronchiseptica showed excellent enantioselectivity (>99% ee) and yield (>91%) in the desymmetrisation of prochiral malonates. Conclusions: We were able to present the proof-of-concept of photoautotrophic enzyme expression as a viable alternative to heterotrophic expression hosts. Our results show that the introduction of foreign genes is straightforward. Cell components from Synechocystis did not interfere with the stereoselective transformations, underlining the usability of photoautotrophic organisms for the production of enzymes. Given the considerable commercial value of recombinant biocatalysts, cyanobacterial enzyme expression has thus the potential to complement existing approaches to use phototrophic organisms for the production of chemicals and biofuels.

KW - Biocatalysis

KW - Enantioselectivity

KW - Enzyme expression

KW - Photosynthesis

KW - Sustainability

UR - http://www.scopus.com/inward/record.url?scp=84928572508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928572508&partnerID=8YFLogxK

U2 - 10.1186/s12934-015-0233-5

DO - 10.1186/s12934-015-0233-5

M3 - Article

VL - 14

JO - Microbial Cell Factories

JF - Microbial Cell Factories

SN - 1475-2859

IS - 1

M1 - 53

ER -