Phototoxicity of Vascular Endothelial Cells Caused by Contact with Talaporfin Sodium for 15-120 Min: In Vitro and in Vivo Studies

Risa Hamada, Emiyu Ogawa, Tsunenori Arai

Research output: Contribution to journalArticle

Abstract

Objective: To reveal the mechanism of vascular patency in the myocardium after photosensitization immediately after talaporfin sodium (TS) injection in a canine model, we investigated acute injury to vascular endothelial cells (VECs) in vitro and in vivo. Background data: There are many reports of vascular shutdown within the target region in photodynamic therapy with TS. Vascular patency within healthy canine myocardium in which a photosensitization reaction starts immediately after injection of TS has been reported. Materials and methods: TS fluorescence in human umbilical vein endothelial cells and cell lethality were measured with drug contact time (DCT) up to 120 min at 20 μg/mL. Dependence of radiant exposure on cell lethality with 60 min DCT was investigated using two albumin concentrations that corresponded to those in plasma and interstices. Irradiation (21 mW/cm) outside the adventitia of canine cervical veins for 167 or 667 sec was emitted through a diffuser probe 30 min after intravenous injection of TS (2.5 mg/kg). Veins were extracted ∼30 min after the reaction and stained with von Willebrand factor. Results: Intracellular fluorescence increased, but not cell lethality, with increasing DCT. Cell lethality increased gradually and reached 100% over 20 J/cm2 in the albumin concentration in the interstices. Normal VECs were found at the acute phase over 20 J/cm2 with a TS concentration in plasma of ∼14 μg/mL in vivo. Conclusions: VEC injury after a photosensitization reaction to healthy tissue shortly after TS injection might be low enough for the blood vessels to be patent.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalPhotomedicine and Laser Surgery
Volume35
Issue number6
DOIs
Publication statusPublished - 2017 Jun 1

Fingerprint

Phototoxic Dermatitis
Endothelial cells
Endothelial Cells
Sodium
Photosensitivity Disorders
Vascular Patency
Canidae
Injections
Blood Vessels
Albumins
Veins
Myocardium
Fluorescence
Pharmaceutical Preparations
Plasmas
Adventitia
Photodynamic therapy
Human Umbilical Vein Endothelial Cells
Photochemotherapy
Wounds and Injuries

Keywords

  • albumin
  • drug contact time
  • photosensitization reaction
  • Vascular endothelial cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Phototoxicity of Vascular Endothelial Cells Caused by Contact with Talaporfin Sodium for 15-120 Min : In Vitro and in Vivo Studies. / Hamada, Risa; Ogawa, Emiyu; Arai, Tsunenori.

In: Photomedicine and Laser Surgery, Vol. 35, No. 6, 01.06.2017, p. 305-310.

Research output: Contribution to journalArticle

@article{4b29012a374542b88d5cf3b3dc5cd2ec,
title = "Phototoxicity of Vascular Endothelial Cells Caused by Contact with Talaporfin Sodium for 15-120 Min: In Vitro and in Vivo Studies",
abstract = "Objective: To reveal the mechanism of vascular patency in the myocardium after photosensitization immediately after talaporfin sodium (TS) injection in a canine model, we investigated acute injury to vascular endothelial cells (VECs) in vitro and in vivo. Background data: There are many reports of vascular shutdown within the target region in photodynamic therapy with TS. Vascular patency within healthy canine myocardium in which a photosensitization reaction starts immediately after injection of TS has been reported. Materials and methods: TS fluorescence in human umbilical vein endothelial cells and cell lethality were measured with drug contact time (DCT) up to 120 min at 20 μg/mL. Dependence of radiant exposure on cell lethality with 60 min DCT was investigated using two albumin concentrations that corresponded to those in plasma and interstices. Irradiation (21 mW/cm) outside the adventitia of canine cervical veins for 167 or 667 sec was emitted through a diffuser probe 30 min after intravenous injection of TS (2.5 mg/kg). Veins were extracted ∼30 min after the reaction and stained with von Willebrand factor. Results: Intracellular fluorescence increased, but not cell lethality, with increasing DCT. Cell lethality increased gradually and reached 100{\%} over 20 J/cm2 in the albumin concentration in the interstices. Normal VECs were found at the acute phase over 20 J/cm2 with a TS concentration in plasma of ∼14 μg/mL in vivo. Conclusions: VEC injury after a photosensitization reaction to healthy tissue shortly after TS injection might be low enough for the blood vessels to be patent.",
keywords = "albumin, drug contact time, photosensitization reaction, Vascular endothelial cell",
author = "Risa Hamada and Emiyu Ogawa and Tsunenori Arai",
year = "2017",
month = "6",
day = "1",
doi = "10.1089/pho.2016.4164",
language = "English",
volume = "35",
pages = "305--310",
journal = "Photomedicine and Laser Surgery",
issn = "1549-5418",
publisher = "Mary Ann Liebert Inc.",
number = "6",

}

TY - JOUR

T1 - Phototoxicity of Vascular Endothelial Cells Caused by Contact with Talaporfin Sodium for 15-120 Min

T2 - In Vitro and in Vivo Studies

AU - Hamada, Risa

AU - Ogawa, Emiyu

AU - Arai, Tsunenori

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Objective: To reveal the mechanism of vascular patency in the myocardium after photosensitization immediately after talaporfin sodium (TS) injection in a canine model, we investigated acute injury to vascular endothelial cells (VECs) in vitro and in vivo. Background data: There are many reports of vascular shutdown within the target region in photodynamic therapy with TS. Vascular patency within healthy canine myocardium in which a photosensitization reaction starts immediately after injection of TS has been reported. Materials and methods: TS fluorescence in human umbilical vein endothelial cells and cell lethality were measured with drug contact time (DCT) up to 120 min at 20 μg/mL. Dependence of radiant exposure on cell lethality with 60 min DCT was investigated using two albumin concentrations that corresponded to those in plasma and interstices. Irradiation (21 mW/cm) outside the adventitia of canine cervical veins for 167 or 667 sec was emitted through a diffuser probe 30 min after intravenous injection of TS (2.5 mg/kg). Veins were extracted ∼30 min after the reaction and stained with von Willebrand factor. Results: Intracellular fluorescence increased, but not cell lethality, with increasing DCT. Cell lethality increased gradually and reached 100% over 20 J/cm2 in the albumin concentration in the interstices. Normal VECs were found at the acute phase over 20 J/cm2 with a TS concentration in plasma of ∼14 μg/mL in vivo. Conclusions: VEC injury after a photosensitization reaction to healthy tissue shortly after TS injection might be low enough for the blood vessels to be patent.

AB - Objective: To reveal the mechanism of vascular patency in the myocardium after photosensitization immediately after talaporfin sodium (TS) injection in a canine model, we investigated acute injury to vascular endothelial cells (VECs) in vitro and in vivo. Background data: There are many reports of vascular shutdown within the target region in photodynamic therapy with TS. Vascular patency within healthy canine myocardium in which a photosensitization reaction starts immediately after injection of TS has been reported. Materials and methods: TS fluorescence in human umbilical vein endothelial cells and cell lethality were measured with drug contact time (DCT) up to 120 min at 20 μg/mL. Dependence of radiant exposure on cell lethality with 60 min DCT was investigated using two albumin concentrations that corresponded to those in plasma and interstices. Irradiation (21 mW/cm) outside the adventitia of canine cervical veins for 167 or 667 sec was emitted through a diffuser probe 30 min after intravenous injection of TS (2.5 mg/kg). Veins were extracted ∼30 min after the reaction and stained with von Willebrand factor. Results: Intracellular fluorescence increased, but not cell lethality, with increasing DCT. Cell lethality increased gradually and reached 100% over 20 J/cm2 in the albumin concentration in the interstices. Normal VECs were found at the acute phase over 20 J/cm2 with a TS concentration in plasma of ∼14 μg/mL in vivo. Conclusions: VEC injury after a photosensitization reaction to healthy tissue shortly after TS injection might be low enough for the blood vessels to be patent.

KW - albumin

KW - drug contact time

KW - photosensitization reaction

KW - Vascular endothelial cell

UR - http://www.scopus.com/inward/record.url?scp=85020435293&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020435293&partnerID=8YFLogxK

U2 - 10.1089/pho.2016.4164

DO - 10.1089/pho.2016.4164

M3 - Article

C2 - 28437192

AN - SCOPUS:85020435293

VL - 35

SP - 305

EP - 310

JO - Photomedicine and Laser Surgery

JF - Photomedicine and Laser Surgery

SN - 1549-5418

IS - 6

ER -