PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

Yuki Hiruta, Takaaki Funatsu, Minami Matsuura, Jian Wang, Eri Ayano, Hideko Kanazawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A pH/temperature-responsive fluorescence polymer, I<inf>42</inf>D<inf>50</inf>S<inf>8</inf>-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I<inf>42</inf>D<inf>50</inf>S<inf>8</inf>-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I<inf>42</inf>D<inf>50</inf>S<inf>8</inf>-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

Original languageEnglish
Pages (from-to)724-731
Number of pages8
JournalSensors and Actuators, B: Chemical
Issue numberPartA
DOIs
Publication statusPublished - 2015

Fingerprint

Polymers
Fluorescence
fluorescence
probes
polymers
carboxyl group
Tumors
fragmentation
tumors
polymerization
Phase transitions
Chemical activation
Polymerization
activation
Imaging techniques
Temperature
temperature
N-isopropylacrylamide
N-hydroxysuccinimide
5-aminofluorescein

Keywords

  • Acidic tumor microenvironment
  • Cellular uptake
  • Fluorescence polymer
  • pH

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Instrumentation

Cite this

PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake. / Hiruta, Yuki; Funatsu, Takaaki; Matsuura, Minami; Wang, Jian; Ayano, Eri; Kanazawa, Hideko.

In: Sensors and Actuators, B: Chemical, No. PartA, 2015, p. 724-731.

Research output: Contribution to journalArticle

Hiruta, Yuki ; Funatsu, Takaaki ; Matsuura, Minami ; Wang, Jian ; Ayano, Eri ; Kanazawa, Hideko. / PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake. In: Sensors and Actuators, B: Chemical. 2015 ; No. PartA. pp. 724-731.
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