Abstract
Using the mitochondrial cytochrome b gene, we investigated phylogenetic relationships between and within the Japanese clawed salamander, Onychodactylus japonicus, and its close continental relative O. fischeri. Monophyly of O. japonicus was well supported, and O. japonicus was clearly distinguished from O. fischeri. However, O. fischeri comprises genetically distinct populations from Russia, NE China, and Korea that do not form a monophyletic group. Within O. japonicus, four major clades were clearly recognized: Clade I from northern Tohoku district, Clade II from southern Tohoku district and the Tsukuba Mountains, Clade III from southwestern Honshu, and Clade IV from Kinki and Chugoku districts in Honshu and from Shikoku. Although genetic distances among these clades were large (5.5-9.6%), relationships among the clades were unresolved. All clades except Clade I contained two or three distinct subclades. In several localities in Kinki and Chugoku, Clades III and IV were sympatric. The estimated divergence times and available geohistorical data suggest that O. japonicus began to differentiate in the Upper Late Miocene and that the pattern of genetic differentiation of this species has been affected strongly by climate changes and geohistorical events such as volcanic activity and mountain formation. Our results suggest that both O. fischeri and O. japonicus comprise multiple cryptic species.
| Original language | English |
|---|---|
| Pages (from-to) | 249-259 |
| Number of pages | 11 |
| Journal | Molecular Phylogenetics and Evolution |
| Volume | 49 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Oct 1 |
| Externally published | Yes |
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Keywords
- Biogeography
- Cytochrome b
- mtDNA
- Onychodactylus fischeri
- Onychodactylus japonicus
- Paraphyly
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Molecular Biology
- Genetics
Cite this
Phylogenetic relationships and biogeography of the Japanese clawed salamander, Onychodactylus japonicus (Amphibia : Caudata: Hynobiidae), and its congener inferred from the mitochondrial cytochrome b gene. / Yoshikawa, Natsuhiko; Matsui, Masafumi; Nishikawa, Kanto; Kim, Jong Bum; Kryukov, Alexei.
In: Molecular Phylogenetics and Evolution, Vol. 49, No. 1, 01.10.2008, p. 249-259.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Phylogenetic relationships and biogeography of the Japanese clawed salamander, Onychodactylus japonicus (Amphibia
T2 - Caudata: Hynobiidae), and its congener inferred from the mitochondrial cytochrome b gene
AU - Yoshikawa, Natsuhiko
AU - Matsui, Masafumi
AU - Nishikawa, Kanto
AU - Kim, Jong Bum
AU - Kryukov, Alexei
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Using the mitochondrial cytochrome b gene, we investigated phylogenetic relationships between and within the Japanese clawed salamander, Onychodactylus japonicus, and its close continental relative O. fischeri. Monophyly of O. japonicus was well supported, and O. japonicus was clearly distinguished from O. fischeri. However, O. fischeri comprises genetically distinct populations from Russia, NE China, and Korea that do not form a monophyletic group. Within O. japonicus, four major clades were clearly recognized: Clade I from northern Tohoku district, Clade II from southern Tohoku district and the Tsukuba Mountains, Clade III from southwestern Honshu, and Clade IV from Kinki and Chugoku districts in Honshu and from Shikoku. Although genetic distances among these clades were large (5.5-9.6%), relationships among the clades were unresolved. All clades except Clade I contained two or three distinct subclades. In several localities in Kinki and Chugoku, Clades III and IV were sympatric. The estimated divergence times and available geohistorical data suggest that O. japonicus began to differentiate in the Upper Late Miocene and that the pattern of genetic differentiation of this species has been affected strongly by climate changes and geohistorical events such as volcanic activity and mountain formation. Our results suggest that both O. fischeri and O. japonicus comprise multiple cryptic species.
AB - Using the mitochondrial cytochrome b gene, we investigated phylogenetic relationships between and within the Japanese clawed salamander, Onychodactylus japonicus, and its close continental relative O. fischeri. Monophyly of O. japonicus was well supported, and O. japonicus was clearly distinguished from O. fischeri. However, O. fischeri comprises genetically distinct populations from Russia, NE China, and Korea that do not form a monophyletic group. Within O. japonicus, four major clades were clearly recognized: Clade I from northern Tohoku district, Clade II from southern Tohoku district and the Tsukuba Mountains, Clade III from southwestern Honshu, and Clade IV from Kinki and Chugoku districts in Honshu and from Shikoku. Although genetic distances among these clades were large (5.5-9.6%), relationships among the clades were unresolved. All clades except Clade I contained two or three distinct subclades. In several localities in Kinki and Chugoku, Clades III and IV were sympatric. The estimated divergence times and available geohistorical data suggest that O. japonicus began to differentiate in the Upper Late Miocene and that the pattern of genetic differentiation of this species has been affected strongly by climate changes and geohistorical events such as volcanic activity and mountain formation. Our results suggest that both O. fischeri and O. japonicus comprise multiple cryptic species.
KW - Biogeography
KW - Cytochrome b
KW - mtDNA
KW - Onychodactylus fischeri
KW - Onychodactylus japonicus
KW - Paraphyly
UR - http://www.scopus.com/inward/record.url?scp=52049110036&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52049110036&partnerID=8YFLogxK
U2 - 10.1016/j.ympev.2008.07.016
DO - 10.1016/j.ympev.2008.07.016
M3 - Article
C2 - 18713651
AN - SCOPUS:52049110036
VL - 49
SP - 249
EP - 259
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
SN - 1055-7903
IS - 1
ER -