Phylogenetic relationships of two Salamandrella species as revealed by mitochondrial DNA and allozyme variation (Amphibia: Caudata: Hynobiidae)

Masafumi Matsui, Natsuhiko Yoshikawa, Atsushi Tominaga, Takanori Sato, Sen Takenaka, Shingo Tanabe, Kanto Nishikawa, Shigehiro Nakabayashi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We conducted molecular phylogenetic analyses to confirm taxonomic relationships and to delimit distributional ranges of Siberian salamanders, Salamandrella keyserlingii and Salamandrella schrenckii, and to elucidate the origin of the isolated population of this species complex on Hokkaido, Japan. Phylogenetic trees constructed by MP, NJ, ML, and Bayesian methods, using complete sequences of mitochondrial cytochrome b genes, all indicated monophyly of Salamandrella and of each of the two species. Identical relationships were found on UPGMA, NJ, and CONTML trees derived from electrophoretic analysis of variation in 18 inferred allozyme loci. Populations from Hokkaido and northeastern China proved to be S. keyserlingii, while populations from Khabarovsk and Lazovsky are S. schrenckii. Genetic differentiations of S. keyserlingii within Sakhalin, and between Sakhalin and Hokkaido, are substantial. The Hokkaido population is hypothesized to have been isolated on the island since early Pleistocene, much earlier than isolation of sympatric anuran populations from their Sakhalin relatives. In contrast, the continental populations of S. keyserlingii are only slightly differentiated from some Sakhalin populations, and are thought to have expanded their ranges in the late Pleistocene.

Original languageEnglish
Pages (from-to)84-93
Number of pages10
JournalMolecular Phylogenetics and Evolution
Volume48
Issue number1
DOIs
Publication statusPublished - 2008 Jul 1
Externally publishedYes

Fingerprint

Urodela
Caudata
Amphibia
Amphibians
allozyme
Mitochondrial DNA
allozymes
mitochondrial DNA
Isoenzymes
Pleistocene
phylogenetics
Japan
phylogeny
isolated population
species complex
genetic differentiation
Population
cytochrome
gene
Bayesian theory

Keywords

  • Allozyme
  • Cytochrome b
  • mtDNA
  • Salamandrella keyserlingii
  • Salamandrella schrenckii

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Phylogenetic relationships of two Salamandrella species as revealed by mitochondrial DNA and allozyme variation (Amphibia : Caudata: Hynobiidae). / Matsui, Masafumi; Yoshikawa, Natsuhiko; Tominaga, Atsushi; Sato, Takanori; Takenaka, Sen; Tanabe, Shingo; Nishikawa, Kanto; Nakabayashi, Shigehiro.

In: Molecular Phylogenetics and Evolution, Vol. 48, No. 1, 01.07.2008, p. 84-93.

Research output: Contribution to journalArticle

Matsui, Masafumi ; Yoshikawa, Natsuhiko ; Tominaga, Atsushi ; Sato, Takanori ; Takenaka, Sen ; Tanabe, Shingo ; Nishikawa, Kanto ; Nakabayashi, Shigehiro. / Phylogenetic relationships of two Salamandrella species as revealed by mitochondrial DNA and allozyme variation (Amphibia : Caudata: Hynobiidae). In: Molecular Phylogenetics and Evolution. 2008 ; Vol. 48, No. 1. pp. 84-93.
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