Physical and functional interactions between STAP-2/BKS and STAT5

Yuichi Sekine, Tetsuya Yamamoto, Taro Yumioka, Kenji Sugiyama, Satoshi Tsuji, Kenji Oritani, Kazuya Shimoda, Mayu Minoguchi, Akihiko Yoshimura, Tadashi Matsuda

Research output: Contribution to journalArticle

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Abstract

Signal-transducing adaptor protein family of proteins (STAPs), which currently contains two members, are proposed to be adaptor molecules because of their pleckstrin homology (PH) and Src-homology 2 (SH2)-like domains. STAP-1 has been shown to interact with STAT5 and the tyrosine kinase Tec. With regard to STAP-2/BKS functions, immunoprecipitation experiments and intracellular stainings revealed STAP-2/BKS binds STAT5 in several types of cells. Mutational studies revealed that the PH- and SH2-like domains of STAP-2/BKS interacted with the C-terminal region of STAT5. STAP-2/BKS and STAT5 were found to constitutively co-localize in the cytoplasm of resting cells, but STAP-2/BKS was found to dissociate upon STAT5 phosphorylation, suggesting a role in regulating signaling of STAT5. The physiological role of these interactions is not fully understood, but in studies of overexpression of STAP-2/BKS, cytokine-induced tyrosine phosphorylation and transcriptional activation of STAT5 was diminished. In addition, thymocytes from STAP-2/BKS-deficient mice showed the enhanced interleukin-2-dependent cell growth. Taken together, STAP-2/BKS is an additional modulator of STAT5-mediated signaling.

Original languageEnglish
Pages (from-to)8188-8196
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number9
DOIs
Publication statusPublished - 2005 Mar 4
Externally publishedYes

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Signal Transducing Adaptor Proteins
Proteins
Phosphorylation
Cell growth
Thymocytes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sekine, Y., Yamamoto, T., Yumioka, T., Sugiyama, K., Tsuji, S., Oritani, K., ... Matsuda, T. (2005). Physical and functional interactions between STAP-2/BKS and STAT5. Journal of Biological Chemistry, 280(9), 8188-8196. https://doi.org/10.1074/jbc.M411692200

Physical and functional interactions between STAP-2/BKS and STAT5. / Sekine, Yuichi; Yamamoto, Tetsuya; Yumioka, Taro; Sugiyama, Kenji; Tsuji, Satoshi; Oritani, Kenji; Shimoda, Kazuya; Minoguchi, Mayu; Yoshimura, Akihiko; Matsuda, Tadashi.

In: Journal of Biological Chemistry, Vol. 280, No. 9, 04.03.2005, p. 8188-8196.

Research output: Contribution to journalArticle

Sekine, Y, Yamamoto, T, Yumioka, T, Sugiyama, K, Tsuji, S, Oritani, K, Shimoda, K, Minoguchi, M, Yoshimura, A & Matsuda, T 2005, 'Physical and functional interactions between STAP-2/BKS and STAT5', Journal of Biological Chemistry, vol. 280, no. 9, pp. 8188-8196. https://doi.org/10.1074/jbc.M411692200
Sekine Y, Yamamoto T, Yumioka T, Sugiyama K, Tsuji S, Oritani K et al. Physical and functional interactions between STAP-2/BKS and STAT5. Journal of Biological Chemistry. 2005 Mar 4;280(9):8188-8196. https://doi.org/10.1074/jbc.M411692200
Sekine, Yuichi ; Yamamoto, Tetsuya ; Yumioka, Taro ; Sugiyama, Kenji ; Tsuji, Satoshi ; Oritani, Kenji ; Shimoda, Kazuya ; Minoguchi, Mayu ; Yoshimura, Akihiko ; Matsuda, Tadashi. / Physical and functional interactions between STAP-2/BKS and STAT5. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 9. pp. 8188-8196.
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