Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome

Mitsuhiro Itaya, J. J. Laffan, N. Sueoka

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The precise physical locations of the oriC region and the region for type II DNA binding to the membrane on the Bacillus subtilis 168 chromosome were determined. The DNA regions were physically mapped by creating new restriction sites (NotI and SfiI) within these regions. The physical distance between oriC and the type II DNA-binding region was verified with the creation of a novel sequence cleaved by endonuclease I-SceI in each of the above regions. Complete removal of the defined type II membrane-binding region produced no noticeable phenotype.

Original languageEnglish
Pages (from-to)5466-5470
Number of pages5
JournalJournal of Bacteriology
Volume174
Issue number16
Publication statusPublished - 1992
Externally publishedYes

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Bacillus subtilis
Chromosomes
Membranes
DNA
Deoxyribonuclease I
Phenotype

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome. / Itaya, Mitsuhiro; Laffan, J. J.; Sueoka, N.

In: Journal of Bacteriology, Vol. 174, No. 16, 1992, p. 5466-5470.

Research output: Contribution to journalArticle

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