Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome

M. Itaya, J. J. Laffan, N. Sueoka

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The precise physical locations of the oriC region and the region for type II DNA binding to the membrane on the Bacillus subtilis 168 chromosome were determined. The DNA regions were physically mapped by creating new restriction sites (NotI and SfiI) within these regions. The physical distance between oriC and the type II DNA-binding region was verified with the creation of a novel sequence cleaved by endonuclease I-SceI in each of the above regions. Complete removal of the defined type II membrane-binding region produced no noticeable phenotype.

Original languageEnglish
Pages (from-to)5466-5470
Number of pages5
JournalJournal of Bacteriology
Volume174
Issue number16
DOIs
Publication statusPublished - 1992 Jan 1

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome'. Together they form a unique fingerprint.

  • Cite this