Physical mapping of multiple homologous genes in the Bacillus subtilis 168 chromosome

identification of ten ribosomal RNA operon loci.

Mitsuhiro Itaya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i.e., the NotI and SfiI restriction map [M. Itaya and Tanaka, J. Mol. Biol., 220, 631-648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.

Original languageEnglish
Pages (from-to)1611-1614
Number of pages4
JournalBioscience, biotechnology, and biochemistry
Volume57
Issue number9
Publication statusPublished - 1993 Sep
Externally publishedYes

Fingerprint

Ribosomal RNA
physical chromosome mapping
Bacilli
operon
Operon
Chromosomes
Bacillus subtilis
Genes
ribosomal RNA
chromosomes
loci
Homologous Recombination
homologous recombination
genes
Membranes

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

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