Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual f-bar domain proteins

Yohko Tanaka-Takiguchi, Toshiki Itoh, Kazuya Tsujita, Shunsuke Yamada, Miho Yanagisawa, Kei Fujiwara, Akihisa Yamamoto, Masatoshi Ichikawa, Kingo Takiguchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The Fer-CIP4 homology-BAR (F-BAR) domain, which was identified as a biological membrane-deforming module, has been reported to transform lipid bilayer membranes into tubules. However, details of the tubulation process, the mechanism, and the properties of the generated tubules remain unknown. Here, we successfully monitored the entire process of tubulation and the behavior of elongated tubules caused by four different F-BAR domain family proteins (FBP17, CIP4, PSTPIP1, and Pacsin2) using direct real-time imaging of giant unilamellar liposomes with dark-field optical microscopy. FBP17 and CIP4 develop many protrusions simultaneously over the entire surface of individual liposomes, whereas PSTPIP1 and Pacsin2 develop only a few protrusions from a narrow restricted part of the surface of individual liposomes. Tubules formed by FBP17 or CIP4 have higher bending rigidities than those formed by PSTPIP1 or Pacsin2. The results provide striking evidence that these four F-BAR domain family proteins should be classified into two groups: one group of FBP17 and CIP4 and another group of PSTPIP1 and Pacsin2. This classification is consistent with the phylogenetic proximity among these proteins and suggests that the nature of the respective tubulation is associated with biological function. These findings aid in the quantitative assessment with respect to manipulating the morphology of lipid bilayers using membrane-deforming proteins.

Original languageEnglish
Pages (from-to)328-336
Number of pages9
JournalLangmuir
Volume29
Issue number1
DOIs
Publication statusPublished - 2013 Jan 8
Externally publishedYes

Fingerprint

Liposomes
homology
Lipid bilayers
proteins
Proteins
Imaging techniques
membranes
Biological membranes
lipids
Unilamellar Liposomes
Membrane Lipids
Membranes
Rigidity
Optical microscopy
Membrane Proteins
rigidity
proximity
modules
microscopy

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual f-bar domain proteins. / Tanaka-Takiguchi, Yohko; Itoh, Toshiki; Tsujita, Kazuya; Yamada, Shunsuke; Yanagisawa, Miho; Fujiwara, Kei; Yamamoto, Akihisa; Ichikawa, Masatoshi; Takiguchi, Kingo.

In: Langmuir, Vol. 29, No. 1, 08.01.2013, p. 328-336.

Research output: Contribution to journalArticle

Tanaka-Takiguchi, Y, Itoh, T, Tsujita, K, Yamada, S, Yanagisawa, M, Fujiwara, K, Yamamoto, A, Ichikawa, M & Takiguchi, K 2013, 'Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual f-bar domain proteins', Langmuir, vol. 29, no. 1, pp. 328-336. https://doi.org/10.1021/la303902q
Tanaka-Takiguchi, Yohko ; Itoh, Toshiki ; Tsujita, Kazuya ; Yamada, Shunsuke ; Yanagisawa, Miho ; Fujiwara, Kei ; Yamamoto, Akihisa ; Ichikawa, Masatoshi ; Takiguchi, Kingo. / Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual f-bar domain proteins. In: Langmuir. 2013 ; Vol. 29, No. 1. pp. 328-336.
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