Physiological effects of a habituation procedure for functional MRI in awake mice using a cryogenic radiofrequency probe

Keitaro Yoshida, Yu Mimura, Ryosuke Ishihara, Hiroshi Nishida, Yuji Komaki, Tomohito Minakuchi, Tomokazu Tsurugizawa, Masaru Mimura, Hideyuki Okano, Kenji Tanaka, Norio Takata

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background Functional magnetic resonance imaging (fMRI) in mice is typically performed under anesthesia due to difficulties in holding the head of awake mice stably with a conventional three-point fixation method that uses a tooth-bar and earplugs. Although some studies have succeeded in fMRI in awake mice by attaching a head-post on the skull, this cannot be applied to fMRI using a high signal-to-noise ratio (SNR) cryogenic MRI-detector, CryoProbe, because it covers the head of a mouse closely. New method We developed head-fixation implements for awake mice that are applicable to fMRI using CryoProbe. Results A head-bar was surgically attached to the skull of a mouse that was then habituated to a mock fMRI-environment, two hours/day for eight days with physiological examinations of body-weight, fecal weight, electromyogram (EMG), and electrocardiogram. EMG power decreased with just one day of habituation, whereas heart rate decreased after at least seven days of habituation. Estimated head motions of awake mice during fMRI were significantly smaller than a voxel size. Unexpectedly, temporal SNR of fMRI signals for awake mice was higher than that for anesthetized mice held by a conventional method. Functional connectivity in the brain of both anesthetized and awake mice showed bilateral and unilateral networks. Comparison with existing method(s) fMRI using CryoProbe had been performed on anesthetized mice previously. Our method does not use anesthetics during habituation or fMRI. Conclusion Our method would be beneficial for translational research using fMRI in mice and humans because human fMRI is typically performed without anesthetics.

Original languageEnglish
Pages (from-to)38-48
Number of pages11
JournalJournal of Neuroscience Methods
Volume274
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Magnetic Resonance Imaging
Head
Signal-To-Noise Ratio
Electromyography
Skull
Anesthetics
Ear Protective Devices
Translational Medical Research
Tooth
Electrocardiography
Anesthesia
Heart Rate
Body Weight
Weights and Measures
Brain

Keywords

  • Awake
  • Conscious
  • CryoProbe
  • fMRI
  • Functional connectivity
  • Habituation
  • Mouse
  • Resting-state network

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Physiological effects of a habituation procedure for functional MRI in awake mice using a cryogenic radiofrequency probe. / Yoshida, Keitaro; Mimura, Yu; Ishihara, Ryosuke; Nishida, Hiroshi; Komaki, Yuji; Minakuchi, Tomohito; Tsurugizawa, Tomokazu; Mimura, Masaru; Okano, Hideyuki; Tanaka, Kenji; Takata, Norio.

In: Journal of Neuroscience Methods, Vol. 274, 01.12.2016, p. 38-48.

Research output: Contribution to journalArticle

Yoshida, Keitaro ; Mimura, Yu ; Ishihara, Ryosuke ; Nishida, Hiroshi ; Komaki, Yuji ; Minakuchi, Tomohito ; Tsurugizawa, Tomokazu ; Mimura, Masaru ; Okano, Hideyuki ; Tanaka, Kenji ; Takata, Norio. / Physiological effects of a habituation procedure for functional MRI in awake mice using a cryogenic radiofrequency probe. In: Journal of Neuroscience Methods. 2016 ; Vol. 274. pp. 38-48.
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