Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

Takeshi Suzuki; Ryoichi Takagi; Koichiro Kawashima

doi:10.1016/j.neulet.2003.11.031

Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

Takeshi Suzuki, Ryoichi Takagi, Koichiro Kawashima

School of Pharmacy

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA _A receptor stimulation and the associated increase in Cl^- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABA_A receptor Cl^- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl^- channel increases neuronal excitability even in an early stage of the development.

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Neuroscience Letters
Volume	356
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2003.11.031
Publication status	Published - 2004 Feb 6

Keywords

Acetylcholine
Chloride
Culture
GABA
Glutamic acid decarboxylase
Hippocampus
Septum

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/j.neulet.2003.11.031

Fingerprint Dive into the research topics of 'Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons'. Together they form a unique fingerprint.

Cite this

@article{63f7f803cad645a98923f87845575786,

title = "Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons",

abstract = "GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.",

keywords = "Acetylcholine, Chloride, Culture, GABA, Glutamic acid decarboxylase, Hippocampus, Septum",

author = "Takeshi Suzuki and Ryoichi Takagi and Koichiro Kawashima",

year = "2004",

month = feb,

day = "6",

doi = "10.1016/j.neulet.2003.11.031",

language = "English",

volume = "356",

pages = "57--60",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

AU - Suzuki, Takeshi

AU - Takagi, Ryoichi

AU - Kawashima, Koichiro

PY - 2004/2/6

Y1 - 2004/2/6

N2 - GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.

AB - GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.

KW - Acetylcholine

KW - Chloride

KW - Culture

KW - GABA

KW - Glutamic acid decarboxylase

KW - Hippocampus

KW - Septum

UR - http://www.scopus.com/inward/record.url?scp=1642431685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642431685&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2003.11.031

DO - 10.1016/j.neulet.2003.11.031

M3 - Article

C2 - 14746901

AN - SCOPUS:1642431685

VL - 356

SP - 57

EP - 60

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -