Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

Takeshi Suzuki, Ryoichi Takagi, Koichiro Kawashima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.

Original languageEnglish
Pages (from-to)57-60
Number of pages4
JournalNeuroscience Letters
Volume356
Issue number1
DOIs
Publication statusPublished - 2004 Feb 6

Fingerprint

Picrotoxin
GABA-A Receptors
gamma-Aminobutyric Acid
Acetylcholine
Neurons
Brain
Neurotransmitter Agents
Membranes

Keywords

  • Acetylcholine
  • Chloride
  • Culture
  • GABA
  • Glutamic acid decarboxylase
  • Hippocampus
  • Septum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons. / Suzuki, Takeshi; Takagi, Ryoichi; Kawashima, Koichiro.

In: Neuroscience Letters, Vol. 356, No. 1, 06.02.2004, p. 57-60.

Research output: Contribution to journalArticle

Suzuki, Takeshi ; Takagi, Ryoichi ; Kawashima, Koichiro. / Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons. In: Neuroscience Letters. 2004 ; Vol. 356, No. 1. pp. 57-60.
@article{63f7f803cad645a98923f87845575786,
title = "Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons",
abstract = "GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.",
keywords = "Acetylcholine, Chloride, Culture, GABA, Glutamic acid decarboxylase, Hippocampus, Septum",
author = "Takeshi Suzuki and Ryoichi Takagi and Koichiro Kawashima",
year = "2004",
month = "2",
day = "6",
doi = "10.1016/j.neulet.2003.11.031",
language = "English",
volume = "356",
pages = "57--60",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

AU - Suzuki, Takeshi

AU - Takagi, Ryoichi

AU - Kawashima, Koichiro

PY - 2004/2/6

Y1 - 2004/2/6

N2 - GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.

AB - GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.

KW - Acetylcholine

KW - Chloride

KW - Culture

KW - GABA

KW - Glutamic acid decarboxylase

KW - Hippocampus

KW - Septum

UR - http://www.scopus.com/inward/record.url?scp=1642431685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642431685&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2003.11.031

DO - 10.1016/j.neulet.2003.11.031

M3 - Article

C2 - 14746901

AN - SCOPUS:1642431685

VL - 356

SP - 57

EP - 60

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -