Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

Takeshi Suzuki; Ryoichi Takagi; Koichiro Kawashima

doi:10.1016/j.neulet.2003.11.031

Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

Takeshi Suzuki, Ryoichi Takagi, Koichiro Kawashima

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA _A receptor stimulation and the associated increase in Cl^- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABA_A receptor Cl^- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl^- channel increases neuronal excitability even in an early stage of the development.

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Neuroscience Letters
Volume	356
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2003.11.031
Publication status	Published - 2004 Feb 6

Keywords

Acetylcholine
Chloride
Culture
GABA
Glutamic acid decarboxylase
Hippocampus
Septum

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neulet.2003.11.031

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abstract = "GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.",

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author = "Takeshi Suzuki and Ryoichi Takagi and Koichiro Kawashima",

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AU - Suzuki, Takeshi

AU - Takagi, Ryoichi

AU - Kawashima, Koichiro

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AB - GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA A receptor stimulation and the associated increase in Cl- conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABAA receptor Cl- channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl- channel increases neuronal excitability even in an early stage of the development.

KW - Acetylcholine

KW - Chloride

KW - Culture

KW - GABA

KW - Glutamic acid decarboxylase

KW - Hippocampus

KW - Septum

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Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

Abstract

Keywords

ASJC Scopus subject areas

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