Pigment epithelium-derived factor inhibits vascular endothelial growth factor-induced vascular hyperpermeability both in vitro and in vivo

Sho Ichi Yamagishi, R. Abe, Y. Jinnouchi, T. Matsui, T. Imaizumi, Hiroyoshi Inoue

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Administration of pigment epithelium-derived factor (PEDF) inhibits advanced glycation end products-elicited retinal vascular hyperpermeability, as well as cold injury-induced brain oedema in rats. However, the underlying molecular mechanism by which PEDF blocks the hyperpermeability in vivo is not fully understood. This study investigated whether PEDF could inhibit vascular endothelial growth factor (VEGF)-induced vascular hyperpermeability both in vitro and in vivo. The Miles assay revealed that, after intradermal injection of VEGF in nude mice, simultaneous administration of PEDF inhibited vascular hyperpermeability in a dose-dependent manner. The in vitro permeability assay also showed that PEDF blocked the VEGF-induced barrier dysfunction in endothelial cells. These results demonstrated that PEDF could inhibit the VEGF-induced vascular hyperpermeability both in vitro and in vivo, and suggest that PEGF may be suitable to be considered as a novel therapeutic agent for various vasopermeable disorders in which VEGF is involved.

Original languageEnglish
Pages (from-to)896-899
Number of pages4
JournalJournal of International Medical Research
Volume35
Issue number6
Publication statusPublished - 2007 Nov
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor A
Blood Vessels
Assays
Intradermal Injections
Retinal Vessels
Advanced Glycosylation End Products
Vascular System Injuries
Brain Edema
Endothelial cells
Nude Mice
In Vitro Techniques
pigment epithelium-derived factor
Rats
Permeability
Brain
Endothelial Cells

Keywords

  • Permeability
  • Pigment epithelium-derived factor (PEDF)
  • Vascular endothelial growth factor (VEGF)

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Pigment epithelium-derived factor inhibits vascular endothelial growth factor-induced vascular hyperpermeability both in vitro and in vivo. / Yamagishi, Sho Ichi; Abe, R.; Jinnouchi, Y.; Matsui, T.; Imaizumi, T.; Inoue, Hiroyoshi.

In: Journal of International Medical Research, Vol. 35, No. 6, 11.2007, p. 896-899.

Research output: Contribution to journalArticle

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AU - Imaizumi, T.

AU - Inoue, Hiroyoshi

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