Pigment-epithelium-derived factor (PEDF) inhibits angiotensin-II-induced vascular endothelial growth factor (VEGF) expression in MOLT-3 T cells through anti-oxidative properties

Sho ichi Yamagishi, Takanori Matsui, Kazuo Nakamura, Takafumi Yoshida, Kyoko Shimizu, Yoshiaki Takegami, Tadamichi Shimizu, Hiroyoshi Inoue, Tsutomu Imaizumi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Angiotensin II (Ang II), the dominant effector of the renin-angiotensin system, elicits numerous inflammatory-proliferative responses, thereby being involved in angiogenesis. T cells play an important role in angiogenesis as well by delivering vascular endothelial growth factor (VEGF) to inflammatory sites. Since we have previously shown that pigment-epithelium-derived factor (PEDF) blocks the Ang II signaling in endothelial cells, we studied here whether PEDF could inhibit the Ang-II-induced VEGF expression in MOLT-3 T and examined the potential mechanism of PEDF inhibitory effects. Ang II significantly up-regulated VEGF mRNA levels in MOLT-3 T cells, which was inhibited by PEDF or olmesartan, an Ang II type 1 receptor blocker. PEDF blocked the Ang-II-induced reactive oxygen species (ROS) generation in MOLT-3 T cells. Furthermore, H2O2 was found to up-regulate VEGF mRNA levels in MOLT-3 T cells in a dose-dependent manner. These results demonstrate that PEDF could inhibit the Ang-II-induced VEGF expression in MOLT-3 T cells via suppression of ROS generation. Blockade by PEDF of VEGF expression in T cells may become a novel therapeutic target for pathological angiogenesis.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalMicrovascular Research
Volume71
Issue number3
DOIs
Publication statusPublished - 2006 May
Externally publishedYes

Fingerprint

T-cells
Angiotensin II
Vascular Endothelial Growth Factor A
T-Lymphocytes
Reactive Oxygen Species
Pathologic Neovascularization
Angiotensin II Type 1 Receptor Blockers
Messenger RNA
Endothelial cells
Angiotensins
Renin-Angiotensin System
pigment epithelium-derived factor
Renin
Up-Regulation
Endothelial Cells

Keywords

  • Angiogenesis
  • Angiotensin II
  • PEDF
  • ROS

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Pigment-epithelium-derived factor (PEDF) inhibits angiotensin-II-induced vascular endothelial growth factor (VEGF) expression in MOLT-3 T cells through anti-oxidative properties. / Yamagishi, Sho ichi; Matsui, Takanori; Nakamura, Kazuo; Yoshida, Takafumi; Shimizu, Kyoko; Takegami, Yoshiaki; Shimizu, Tadamichi; Inoue, Hiroyoshi; Imaizumi, Tsutomu.

In: Microvascular Research, Vol. 71, No. 3, 05.2006, p. 222-226.

Research output: Contribution to journalArticle

Yamagishi, Sho ichi ; Matsui, Takanori ; Nakamura, Kazuo ; Yoshida, Takafumi ; Shimizu, Kyoko ; Takegami, Yoshiaki ; Shimizu, Tadamichi ; Inoue, Hiroyoshi ; Imaizumi, Tsutomu. / Pigment-epithelium-derived factor (PEDF) inhibits angiotensin-II-induced vascular endothelial growth factor (VEGF) expression in MOLT-3 T cells through anti-oxidative properties. In: Microvascular Research. 2006 ; Vol. 71, No. 3. pp. 222-226.
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