Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi-interacting RNAs at their 3′ ends

Kuniaki Saito, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

273 Citations (Scopus)

Abstract

Piwi-interacting RNAs (piRNAs) consist of a germline-specific group of small RNAs derived from distinct intergenic loci in the genome. piRNAs function in silencing selfish transposable elements through binding with the PIWI subfamily proteins of Argonautes. Here we show that piRNAs in Drosophila are 2′-O-methylated at their 3′ ends. Loss of Pimet (piRNA methyltransferase), the Drosophila homolog of Arabidopsis HEN1 methyltransferase for microRNAs (miRNAs), results in loss of 2′-O-methylation of fly piRNAs. Recombinant Pimet shows single-stranded small RNA methylation activity in vitro and interacts with the PIWI proteins within Pimet mutant ovary. These results show that Pimet mediates piRNA 2′-O-methylation in Drosophila.

Original languageEnglish
Pages (from-to)1603-1608
Number of pages6
JournalGenes and Development
Volume21
Issue number13
DOIs
Publication statusPublished - 2007 Jul 1
Externally publishedYes

Fingerprint

Methyltransferases
Methylation
Small Interfering RNA
Drosophila
RNA
DNA Transposable Elements
MicroRNAs
Arabidopsis
Diptera
Ovary
Proteins
Genome

Keywords

  • 2′-O-methylation
  • Drosophila
  • HEN1
  • Pimet
  • piRNA

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi-interacting RNAs at their 3′ ends. / Saito, Kuniaki; Sakaguchi, Yuriko; Suzuki, Takeo; Suzuki, Tsutomu; Siomi, Haruhiko; Siomi, Mikiko C.

In: Genes and Development, Vol. 21, No. 13, 01.07.2007, p. 1603-1608.

Research output: Contribution to journalArticle

Saito, Kuniaki ; Sakaguchi, Yuriko ; Suzuki, Takeo ; Suzuki, Tsutomu ; Siomi, Haruhiko ; Siomi, Mikiko C. / Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi-interacting RNAs at their 3′ ends. In: Genes and Development. 2007 ; Vol. 21, No. 13. pp. 1603-1608.
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