Pirfenidone induces intercellular adhesion molecule-1 (ICAM-1) down- regulation on cultured human synovial fibroblasts

M. Kaneko, H. Inoue, R. Nakazawa, N. Azuma, M. Suzuki, S. Yamauchi, S. B. Margolin, Kazuo Tsubota, I. Saito

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Pirfenidone has been shown to modify some cytokine regulatory actions and inhibit fibroblast biochemical reactions resulting in inhibition of proliferation and collagen matrix synthesis by fibroblast. We have investigated the effect of pirfenidone on the expression of cell adhesion molecules. The synovial fibroblasts were treated with IL-1α in the presence or absence of pirfenidone (range 0-1000 μM), and assayed for the expression of adhesion molecules such as ICAM-1 and endothelial-leucocyte adhesion molecule-1 (E-selectin) by cell ELISA. Pirfenidone significantly down- regulated the expression of ICAM-1 on cultured synovial fibroblasts in a dose-dependent manner. In contrast, expression of E-selectin was not affected. Furthermore, we examined whether pirfenidone affects the cellular binding between cultured lymphocytes and IL-1α-stimulated synovial fibroblasts by in vitro binding assay and found their mutual binding was significantly suppressed in a dose-dependent manner by pirfenidone. It is speculated that down-regulation of ICAM-1 might be one of the novel mechanisms of action of pirfenidone. These data indicate a novel mechanism of action for pirfenidone to reduce the activation of synovial fibroblasts.

Original languageEnglish
Pages (from-to)72-76
Number of pages5
JournalClinical and Experimental Immunology
Volume113
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Intercellular Adhesion Molecule-1
Down-Regulation
Fibroblasts
E-Selectin
Interleukin-1
pirfenidone
Cell Adhesion Molecules
Collagen
Enzyme-Linked Immunosorbent Assay
Lymphocytes
Cytokines

Keywords

  • Cell adhesion molecules
  • ICAM-1
  • Pirfenidone
  • Synovial fibroblasts

ASJC Scopus subject areas

  • Immunology

Cite this

Pirfenidone induces intercellular adhesion molecule-1 (ICAM-1) down- regulation on cultured human synovial fibroblasts. / Kaneko, M.; Inoue, H.; Nakazawa, R.; Azuma, N.; Suzuki, M.; Yamauchi, S.; Margolin, S. B.; Tsubota, Kazuo; Saito, I.

In: Clinical and Experimental Immunology, Vol. 113, No. 1, 1998, p. 72-76.

Research output: Contribution to journalArticle

Kaneko, M. ; Inoue, H. ; Nakazawa, R. ; Azuma, N. ; Suzuki, M. ; Yamauchi, S. ; Margolin, S. B. ; Tsubota, Kazuo ; Saito, I. / Pirfenidone induces intercellular adhesion molecule-1 (ICAM-1) down- regulation on cultured human synovial fibroblasts. In: Clinical and Experimental Immunology. 1998 ; Vol. 113, No. 1. pp. 72-76.
@article{194dcbe224ef40b59765ce21c1d85520,
title = "Pirfenidone induces intercellular adhesion molecule-1 (ICAM-1) down- regulation on cultured human synovial fibroblasts",
abstract = "Pirfenidone has been shown to modify some cytokine regulatory actions and inhibit fibroblast biochemical reactions resulting in inhibition of proliferation and collagen matrix synthesis by fibroblast. We have investigated the effect of pirfenidone on the expression of cell adhesion molecules. The synovial fibroblasts were treated with IL-1α in the presence or absence of pirfenidone (range 0-1000 μM), and assayed for the expression of adhesion molecules such as ICAM-1 and endothelial-leucocyte adhesion molecule-1 (E-selectin) by cell ELISA. Pirfenidone significantly down- regulated the expression of ICAM-1 on cultured synovial fibroblasts in a dose-dependent manner. In contrast, expression of E-selectin was not affected. Furthermore, we examined whether pirfenidone affects the cellular binding between cultured lymphocytes and IL-1α-stimulated synovial fibroblasts by in vitro binding assay and found their mutual binding was significantly suppressed in a dose-dependent manner by pirfenidone. It is speculated that down-regulation of ICAM-1 might be one of the novel mechanisms of action of pirfenidone. These data indicate a novel mechanism of action for pirfenidone to reduce the activation of synovial fibroblasts.",
keywords = "Cell adhesion molecules, ICAM-1, Pirfenidone, Synovial fibroblasts",
author = "M. Kaneko and H. Inoue and R. Nakazawa and N. Azuma and M. Suzuki and S. Yamauchi and Margolin, {S. B.} and Kazuo Tsubota and I. Saito",
year = "1998",
doi = "10.1046/j.1365-2249.1998.00618.x",
language = "English",
volume = "113",
pages = "72--76",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Pirfenidone induces intercellular adhesion molecule-1 (ICAM-1) down- regulation on cultured human synovial fibroblasts

AU - Kaneko, M.

AU - Inoue, H.

AU - Nakazawa, R.

AU - Azuma, N.

AU - Suzuki, M.

AU - Yamauchi, S.

AU - Margolin, S. B.

AU - Tsubota, Kazuo

AU - Saito, I.

PY - 1998

Y1 - 1998

N2 - Pirfenidone has been shown to modify some cytokine regulatory actions and inhibit fibroblast biochemical reactions resulting in inhibition of proliferation and collagen matrix synthesis by fibroblast. We have investigated the effect of pirfenidone on the expression of cell adhesion molecules. The synovial fibroblasts were treated with IL-1α in the presence or absence of pirfenidone (range 0-1000 μM), and assayed for the expression of adhesion molecules such as ICAM-1 and endothelial-leucocyte adhesion molecule-1 (E-selectin) by cell ELISA. Pirfenidone significantly down- regulated the expression of ICAM-1 on cultured synovial fibroblasts in a dose-dependent manner. In contrast, expression of E-selectin was not affected. Furthermore, we examined whether pirfenidone affects the cellular binding between cultured lymphocytes and IL-1α-stimulated synovial fibroblasts by in vitro binding assay and found their mutual binding was significantly suppressed in a dose-dependent manner by pirfenidone. It is speculated that down-regulation of ICAM-1 might be one of the novel mechanisms of action of pirfenidone. These data indicate a novel mechanism of action for pirfenidone to reduce the activation of synovial fibroblasts.

AB - Pirfenidone has been shown to modify some cytokine regulatory actions and inhibit fibroblast biochemical reactions resulting in inhibition of proliferation and collagen matrix synthesis by fibroblast. We have investigated the effect of pirfenidone on the expression of cell adhesion molecules. The synovial fibroblasts were treated with IL-1α in the presence or absence of pirfenidone (range 0-1000 μM), and assayed for the expression of adhesion molecules such as ICAM-1 and endothelial-leucocyte adhesion molecule-1 (E-selectin) by cell ELISA. Pirfenidone significantly down- regulated the expression of ICAM-1 on cultured synovial fibroblasts in a dose-dependent manner. In contrast, expression of E-selectin was not affected. Furthermore, we examined whether pirfenidone affects the cellular binding between cultured lymphocytes and IL-1α-stimulated synovial fibroblasts by in vitro binding assay and found their mutual binding was significantly suppressed in a dose-dependent manner by pirfenidone. It is speculated that down-regulation of ICAM-1 might be one of the novel mechanisms of action of pirfenidone. These data indicate a novel mechanism of action for pirfenidone to reduce the activation of synovial fibroblasts.

KW - Cell adhesion molecules

KW - ICAM-1

KW - Pirfenidone

KW - Synovial fibroblasts

UR - http://www.scopus.com/inward/record.url?scp=0031820998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031820998&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2249.1998.00618.x

DO - 10.1046/j.1365-2249.1998.00618.x

M3 - Article

C2 - 9697986

AN - SCOPUS:0031820998

VL - 113

SP - 72

EP - 76

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 1

ER -