Abstract
The acrosome reaction (AR) is a fundamental event for fertilization, which is induced in concert with acrosome reaction-inducing substance (ARIS) and asterosap, both of which are components of starfish egg jelly (EJ During the AR, a spermatozoon undergoes a series of physiological changes, such as in intracellular cGMP concentration ([cGMP]}), pHj and intracellular Ca 2+ concentration ([Ca2+]i). Affinity purification of cGMP-binding protein resulted in the isolation of a regulatory subunit of the cAMP-dependent protein kinase A (PKA), suggesting the involvement of a cAMP-dependent pathway in the AR. By using a cAMP enzyme immunoassay, [cAMP]; was found to increase in starfish spermatozoa when stimulated with ARIS and asterosap. ARIS could also increase the [cAMP]; in the presence of high pH seawater. Pretreatment of spermatozoa with two specific and cell-permeable PKA inhibitors, H89 and KT5720, prevented the induction of the AR in a concentration-dependent manner. These results suggest that PKA activity participates in the induction of the AR with ARIS and asterosap. To investigate this, we have cloned a gene that encodes a regulatory subunit of PKA that had been identified in starfish spermatozoa.
| Original language | English |
|---|---|
| Pages (from-to) | 329-340 |
| Number of pages | 12 |
| Journal | Zygote |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2006 Nov |
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Keywords
- Acrosome reaction
- Cyclic AMP
- Egg jelly
- PKA
- Starfish
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
PKA activation in concert with ARIS and asterosap induces the acrosome reaction in starfish. / Sadiqul Islam, M.; Kawase, O.; Hase, S.; Hoshi, M.; Matsumoto, Midori.
In: Zygote, Vol. 14, No. 4, 11.2006, p. 329-340.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PKA activation in concert with ARIS and asterosap induces the acrosome reaction in starfish
AU - Sadiqul Islam, M.
AU - Kawase, O.
AU - Hase, S.
AU - Hoshi, M.
AU - Matsumoto, Midori
PY - 2006/11
Y1 - 2006/11
N2 - The acrosome reaction (AR) is a fundamental event for fertilization, which is induced in concert with acrosome reaction-inducing substance (ARIS) and asterosap, both of which are components of starfish egg jelly (EJ During the AR, a spermatozoon undergoes a series of physiological changes, such as in intracellular cGMP concentration ([cGMP]}), pHj and intracellular Ca 2+ concentration ([Ca2+]i). Affinity purification of cGMP-binding protein resulted in the isolation of a regulatory subunit of the cAMP-dependent protein kinase A (PKA), suggesting the involvement of a cAMP-dependent pathway in the AR. By using a cAMP enzyme immunoassay, [cAMP]; was found to increase in starfish spermatozoa when stimulated with ARIS and asterosap. ARIS could also increase the [cAMP]; in the presence of high pH seawater. Pretreatment of spermatozoa with two specific and cell-permeable PKA inhibitors, H89 and KT5720, prevented the induction of the AR in a concentration-dependent manner. These results suggest that PKA activity participates in the induction of the AR with ARIS and asterosap. To investigate this, we have cloned a gene that encodes a regulatory subunit of PKA that had been identified in starfish spermatozoa.
AB - The acrosome reaction (AR) is a fundamental event for fertilization, which is induced in concert with acrosome reaction-inducing substance (ARIS) and asterosap, both of which are components of starfish egg jelly (EJ During the AR, a spermatozoon undergoes a series of physiological changes, such as in intracellular cGMP concentration ([cGMP]}), pHj and intracellular Ca 2+ concentration ([Ca2+]i). Affinity purification of cGMP-binding protein resulted in the isolation of a regulatory subunit of the cAMP-dependent protein kinase A (PKA), suggesting the involvement of a cAMP-dependent pathway in the AR. By using a cAMP enzyme immunoassay, [cAMP]; was found to increase in starfish spermatozoa when stimulated with ARIS and asterosap. ARIS could also increase the [cAMP]; in the presence of high pH seawater. Pretreatment of spermatozoa with two specific and cell-permeable PKA inhibitors, H89 and KT5720, prevented the induction of the AR in a concentration-dependent manner. These results suggest that PKA activity participates in the induction of the AR with ARIS and asterosap. To investigate this, we have cloned a gene that encodes a regulatory subunit of PKA that had been identified in starfish spermatozoa.
KW - Acrosome reaction
KW - Cyclic AMP
KW - Egg jelly
KW - PKA
KW - Starfish
UR - http://www.scopus.com/inward/record.url?scp=33845342555&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845342555&partnerID=8YFLogxK
U2 - 10.1017/S0967199406003881
DO - 10.1017/S0967199406003881
M3 - Article
C2 - 17266791
AN - SCOPUS:33845342555
VL - 14
SP - 329
EP - 340
JO - Zygote
JF - Zygote
SN - 0967-1994
IS - 4
ER -