PKA activation in concert with ARIS and asterosap induces the acrosome reaction in starfish

M. Sadiqul Islam, O. Kawase, S. Hase, M. Hoshi, M. Matsumoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The acrosome reaction (AR) is a fundamental event for fertilization, which is induced in concert with acrosome reaction-inducing substance (ARIS) and asterosap, both of which are components of starfish egg jelly (EJ During the AR, a spermatozoon undergoes a series of physiological changes, such as in intracellular cGMP concentration ([cGMP]}), pHj and intracellular Ca 2+ concentration ([Ca2+]i). Affinity purification of cGMP-binding protein resulted in the isolation of a regulatory subunit of the cAMP-dependent protein kinase A (PKA), suggesting the involvement of a cAMP-dependent pathway in the AR. By using a cAMP enzyme immunoassay, [cAMP]; was found to increase in starfish spermatozoa when stimulated with ARIS and asterosap. ARIS could also increase the [cAMP]; in the presence of high pH seawater. Pretreatment of spermatozoa with two specific and cell-permeable PKA inhibitors, H89 and KT5720, prevented the induction of the AR in a concentration-dependent manner. These results suggest that PKA activity participates in the induction of the AR with ARIS and asterosap. To investigate this, we have cloned a gene that encodes a regulatory subunit of PKA that had been identified in starfish spermatozoa.

Original languageEnglish
Pages (from-to)329-340
Number of pages12
JournalZygote
Volume14
Issue number4
DOIs
Publication statusPublished - 2006 Nov 1

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Keywords

  • Acrosome reaction
  • Cyclic AMP
  • Egg jelly
  • PKA
  • Starfish

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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