Planar integrated optical waveguide used as a transducer to yield chemical information

Detection of the activity of proteolytic enzymes e.g. serine-proteases

Gleb Zhylyak, Victor Ramoz-Perez, Michael Linnhoff, Thomas Hug, Daniel Citterio, Ursula E. Spichiger-Keller

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The paper shows the very first results of a feasibility study where the activity of proteolytic enzymes towards dye-labelled artificial substrates immobilized on the surface of planar optical Ta2O5 waveguide was investigated. Within this project, a chromophore label was developed, synthesized and attached to the carboxy-terminus of specific tripeptides. The goal was to develop a highly sensitive optical assay in order to monitor the activity of serine-proteases by cleavage of the amide bond between peptide and chromophore. On the one hand, a strategy was developed to immobilize the labeled tripeptide unto integrated planar waveguides. On the other hand, an instrument, the so-called "chip-reader" was developed to detect the biological process on the surface of the integrated planar optical waveguide. Surface characteristics were analyzed by XPS, TOF-SIMS and contact angle measurements. A comparison between the effectivity of ATR-photometry on chip using TE0 mode and photometry in transmission mode is discussed.

Original languageEnglish
Pages (from-to)603-617
Number of pages15
JournalOptics and Lasers in Engineering
Volume43
Issue number3-5
DOIs
Publication statusPublished - 2005 Mar
Externally publishedYes

Fingerprint

protease
Planar waveguides
Serine Proteases
Optical waveguides
optical waveguides
enzymes
Photometry
Transducers
transducers
Peptide Hydrolases
Chromophores
chromophores
photometry
chips
readers
Angle measurement
Secondary ion mass spectrometry
Amides
secondary ion mass spectrometry
Peptides

Keywords

  • Absorbance measurements
  • Absorption wavelength >630 nm
  • ATR-measurements in the rarer medium
  • Cancer diagnostics
  • Detection in human blood
  • Evanescent field
  • Multiplexing
  • Screening of tumorstatic activity
  • Tracing of endotoxin

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

Cite this

Planar integrated optical waveguide used as a transducer to yield chemical information : Detection of the activity of proteolytic enzymes e.g. serine-proteases. / Zhylyak, Gleb; Ramoz-Perez, Victor; Linnhoff, Michael; Hug, Thomas; Citterio, Daniel; Spichiger-Keller, Ursula E.

In: Optics and Lasers in Engineering, Vol. 43, No. 3-5, 03.2005, p. 603-617.

Research output: Contribution to journalArticle

Zhylyak, Gleb ; Ramoz-Perez, Victor ; Linnhoff, Michael ; Hug, Thomas ; Citterio, Daniel ; Spichiger-Keller, Ursula E. / Planar integrated optical waveguide used as a transducer to yield chemical information : Detection of the activity of proteolytic enzymes e.g. serine-proteases. In: Optics and Lasers in Engineering. 2005 ; Vol. 43, No. 3-5. pp. 603-617.
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