Plant Mucilages. XXXV. Isolation and Characterization of a Mucous Polysaccharide, Hippeastrum-H-glucomannan, from the Bulbs of Hippeastrum hybridum

Masashi Tomoda, Noriko Shimizu, Kazuyo Shimada, Ryoko Gonda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A mucous polysaccharide, named Hippeastrum-H-glucomannan, was isolated from the bulbs of Hippeastrum hybridum Hort. The final preparation was homogeneous as determined by ultracentrifugal analysis, glass-fiber electrophoresis, and gel chromatography. It was composed of D-mannose and D-glucose in the molar ratio of 5:2, and its molecular weight was estimated to be 331000. 0-Acetyl groups were identified and their content amounted to 13.2%. They were located at positions 2, 6 of some of the D-mannose units. Methylation and partial acid hydrolysis studies showed that the glucomannan is mainly composed of β-1 → 4–linked aldohexopyranose residues, and that it contains about eighty-three aldohexose units per six non-reducing groups on average. Both D-mannose and D-glucose units occupy non-reducing terminal positions and branching points linked through position 3.

Original languageEnglish
Pages (from-to)48-51
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume33
Issue number1
DOIs
Publication statusPublished - 1985
Externally publishedYes

Fingerprint

Plant Mucilage
Mannose
Polysaccharides
Glucose
Methylation
Chromatography
Electrophoresis
Gel Chromatography
Hydrolysis
Molecular Weight
Gels
Molecular weight
Acids
(1-6)-alpha-glucomannan
Amaryllidaceae

Keywords

  • acetyl-rich polysaccharide
  • bulb
  • Hippeastrum hybridum
  • Hippeastrum-H-glucomannan
  • intrinsic viscosity
  • molecular weight
  • structure 2,6-di-O-acetyl group

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

Cite this

Plant Mucilages. XXXV. Isolation and Characterization of a Mucous Polysaccharide, Hippeastrum-H-glucomannan, from the Bulbs of Hippeastrum hybridum. / Tomoda, Masashi; Shimizu, Noriko; Shimada, Kazuyo; Gonda, Ryoko.

In: Chemical and Pharmaceutical Bulletin, Vol. 33, No. 1, 1985, p. 48-51.

Research output: Contribution to journalArticle

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AU - Gonda, Ryoko

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