Plasmids captured in C. metallidurans CH34: Defining the PromA family of broad-host-range plasmids

Géraldine A. Van der Auwera, Jaroslaw E. Król, Haruo Suzuki, Brian Foster, Rob Van Houdt, Celeste J. Brown, Max Mergeay, Eva M. Top

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The self-transmissible, broad-host-range (BHR) plasmid pMOL98 was previously isolated from polluted soil using a triparental plasmid capture approach and shown to possess a replicon similar to that of the BHR plasmids pSB102 and pIPO2. Here, complete sequence analysis and comparative genomics reveal that the 55.5 kb nucleotide sequence of pMOL98 shows extensive sequence similarity and synteny with the BHR plasmid family that now includes pIPO2, pSB102, pTER331, and pMRAD02. They share a plasmid backbone comprising replication, partitioning and conjugative transfer functions. Comparison of the variable accessory regions of these plasmids shows that the majority of natural transposons, as well as the mini-transposon used to mark the plasmids, are inserted in the parA locus. The transposon unique to pMOL98 appears to have inserted from the chromosome of the recipient strain used in the plasmid capture procedure. This demonstrates the necessity for careful screening of plasmids and host chromosomes to avoid mis-interpretation of plasmid genome content. The presence of very similar BHR plasmids with different accessory genes in geographically distinct locations suggests an important role in horizontal gene exchange and bacterial adaptation for this recently defined plasmid group, which we propose to name "PromA".

Original languageEnglish
Pages (from-to)193-204
Number of pages12
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume96
Issue number2 SPEC. ISS.
DOIs
Publication statusPublished - 2009
Externally publishedYes

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Host Specificity
Plasmids
Chromosomes
Synteny
Bacterial Genes
Replicon
Genomics
Names
Sequence Analysis
Soil
Genome

Keywords

  • Broad host range
  • Horizontal gene transfer
  • Plasmid
  • Transposon

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Plasmids captured in C. metallidurans CH34 : Defining the PromA family of broad-host-range plasmids. / Van der Auwera, Géraldine A.; Król, Jaroslaw E.; Suzuki, Haruo; Foster, Brian; Van Houdt, Rob; Brown, Celeste J.; Mergeay, Max; Top, Eva M.

In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, Vol. 96, No. 2 SPEC. ISS., 2009, p. 193-204.

Research output: Contribution to journalArticle

Van der Auwera, Géraldine A. ; Król, Jaroslaw E. ; Suzuki, Haruo ; Foster, Brian ; Van Houdt, Rob ; Brown, Celeste J. ; Mergeay, Max ; Top, Eva M. / Plasmids captured in C. metallidurans CH34 : Defining the PromA family of broad-host-range plasmids. In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 2009 ; Vol. 96, No. 2 SPEC. ISS. pp. 193-204.
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