Platelet-activating factor and granulocyte-mediated oxidative stress. Strategy for in vivo oxyradical visualization

Makoto Suematsu, Masaharu Tsuchiya

Research output: Contribution to journalReview articlepeer-review

10 Citations (Scopus)

Abstract

Platelet-activating factor (PAF) and leukotriene B4 are potent lipid mediators of endothelium-granulocyte interaction which results in granulocyte-dependent increased microvascular permeability. The specific mechanism by which PAF induces granulocyte-mediated endothelial injury has not been fully investigated. Digital imaging photonic intensified microscopy has revealed that PAF effectively induces granulocyte-mediated oxidative stress on microvascular beds. The method has made it possible to visualize luminol-dependent photonic burst released from PAF-treated microvascular beds in the rat mesentery. The photonic activities clearly corresponded to the localization of sticking granulocytes in post-capillary venules. By contrast, no significant chemilumigenic response could be detected in the leukotriene B4-induced activation of endothelium-granulocyte interaction in vivo. The present findings suggest that oxygen radicals are not prerequisites for granulocyte adhesion in the microcirculation and provide evidence for a dissociation of in vivo granulocyte function between leukotactic and oxidative activation in leukotriene B4-induced microvascular changes.

Original languageEnglish
Pages (from-to)1362-1368
Number of pages7
JournalLipids
Volume26
Issue number12
DOIs
Publication statusPublished - 1991 Dec 1

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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