Abstract
Early pregnancy factor, a glycoprotein, and platelet activating factor, a phospholipid, are early signals of pregnancy appearing during the preimplantation period. The aim of this study was to determine if the administration of platelet activating factor to rabbits would stimulate the production of early pregnancy factor and, if so, to ascertain the organs responsible. Early pregnancy factor was assayed after the administration of synthetic platelet activating factor to intact rabbits, rabbits that had undergone bilateral salpingo-oophorectomy, and isolated in vitro perfused rabbit ovary and/or oviduct. Although serum early pregnancy factor activity was detectable 30 minutes after platelet activating factor injection in intact rabbits, no activity was found in rabbits whose ovaries and oviducts had been removed. Perfusate early pregnancy factor activity was detectable only in the presence of both ovary and oviduct, and was first evident 120 minutes after platelet activating factor exposure. However, combined samples of perfusates from the ovary alone and oviduct alone expressed positive early pregnancy factor activity. Furthemore, the addition of platelet activating factor to either single or combined perfusates did not increase early pregnancy factor activity. Our data imply that platelet activating factor induces the production of ovary and oviduct early pregnancy factor components independently. (AM J Obstet GYNECOL 1988;159:1580-4.).
| Original language | English |
|---|---|
| Pages (from-to) | 1580-1584 |
| Number of pages | 5 |
| Journal | American Journal of Obstetrics and Gynecology |
| Volume | 159 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1988 |
| Externally published | Yes |
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Keywords
- Early pregnancy factor
- ovary
- oviduct
- perfusion
- platelet activating factor
ASJC Scopus subject areas
- Medicine(all)
- Obstetrics and Gynaecology
Cite this
Platelet activating factor-induced early pregnancy factor activity from the perfused rabbit ovary and oviduct. / Sueoka, Kou; Dharmarajan, A. M.; Miyazaki, Toyohiko; Atlas, Susan J.; Wallach, Edward E.
In: American Journal of Obstetrics and Gynecology, Vol. 159, No. 6, 1988, p. 1580-1584.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Platelet activating factor-induced early pregnancy factor activity from the perfused rabbit ovary and oviduct
AU - Sueoka, Kou
AU - Dharmarajan, A. M.
AU - Miyazaki, Toyohiko
AU - Atlas, Susan J.
AU - Wallach, Edward E.
PY - 1988
Y1 - 1988
N2 - Early pregnancy factor, a glycoprotein, and platelet activating factor, a phospholipid, are early signals of pregnancy appearing during the preimplantation period. The aim of this study was to determine if the administration of platelet activating factor to rabbits would stimulate the production of early pregnancy factor and, if so, to ascertain the organs responsible. Early pregnancy factor was assayed after the administration of synthetic platelet activating factor to intact rabbits, rabbits that had undergone bilateral salpingo-oophorectomy, and isolated in vitro perfused rabbit ovary and/or oviduct. Although serum early pregnancy factor activity was detectable 30 minutes after platelet activating factor injection in intact rabbits, no activity was found in rabbits whose ovaries and oviducts had been removed. Perfusate early pregnancy factor activity was detectable only in the presence of both ovary and oviduct, and was first evident 120 minutes after platelet activating factor exposure. However, combined samples of perfusates from the ovary alone and oviduct alone expressed positive early pregnancy factor activity. Furthemore, the addition of platelet activating factor to either single or combined perfusates did not increase early pregnancy factor activity. Our data imply that platelet activating factor induces the production of ovary and oviduct early pregnancy factor components independently. (AM J Obstet GYNECOL 1988;159:1580-4.).
AB - Early pregnancy factor, a glycoprotein, and platelet activating factor, a phospholipid, are early signals of pregnancy appearing during the preimplantation period. The aim of this study was to determine if the administration of platelet activating factor to rabbits would stimulate the production of early pregnancy factor and, if so, to ascertain the organs responsible. Early pregnancy factor was assayed after the administration of synthetic platelet activating factor to intact rabbits, rabbits that had undergone bilateral salpingo-oophorectomy, and isolated in vitro perfused rabbit ovary and/or oviduct. Although serum early pregnancy factor activity was detectable 30 minutes after platelet activating factor injection in intact rabbits, no activity was found in rabbits whose ovaries and oviducts had been removed. Perfusate early pregnancy factor activity was detectable only in the presence of both ovary and oviduct, and was first evident 120 minutes after platelet activating factor exposure. However, combined samples of perfusates from the ovary alone and oviduct alone expressed positive early pregnancy factor activity. Furthemore, the addition of platelet activating factor to either single or combined perfusates did not increase early pregnancy factor activity. Our data imply that platelet activating factor induces the production of ovary and oviduct early pregnancy factor components independently. (AM J Obstet GYNECOL 1988;159:1580-4.).
KW - Early pregnancy factor
KW - ovary
KW - oviduct
KW - perfusion
KW - platelet activating factor
UR - http://www.scopus.com/inward/record.url?scp=0024261828&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024261828&partnerID=8YFLogxK
U2 - 10.1016/0002-9378(88)90598-4
DO - 10.1016/0002-9378(88)90598-4
M3 - Article
C2 - 3207134
AN - SCOPUS:0024261828
VL - 159
SP - 1580
EP - 1584
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
SN - 0002-9378
IS - 6
ER -