Platelet-Derived Stromal Cell-Derived Factor-1 Is Required for the Transformation of Circulating Monocytes into Multipotential Cells

Noriyuki Seta, Yuka Okazaki, Hiroshi Miyazaki, Takashi Kato, Masataka Kuwana

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background:We previously described a primitive cell population derived from human circulating CD14+ monocytes, named monocyte-derived multipotential cells (MOMCs), which are capable of differentiating into mesenchymal and endothelial lineages. To generate MOMCs in vitro, monocytes are required to bind to fibronectin and be exposed to soluble factor(s) derived from circulating CD14- cells. The present study was conducted to identify factors that induce MOMC differentiation.Methods:We cultured CD14+ monocytes on fibronectin in the presence or absence of platelets, CD14- peripheral blood mononuclear cells, platelet-conditioned medium, or candidate MOMC differentiation factors. The transformation of monocytes into MOMCs was assessed by the presence of spindle-shaped adherent cells, CD34 expression, and the potential to differentiate in vitro into mesenchymal and endothelial lineages.Results:The presence of platelets or platelet-conditioned medium was required to generate MOMCs from monocytes. A screening of candidate platelet-derived soluble factors identified stromal cell-derived factor (SDF)-1 as a requirement for generating MOMCs. Blocking an interaction between SDF-1 and its receptor CXCR4 inhibited MOMC generation, further confirming SDF-1′s critical role in this process. Finally, circulating MOMC precursors were found to reside in the CD14+CXCR4high cell population.Conclusion:The interaction of SDF-1 with CXCR4 is essential for the transformation of circulating monocytes into MOMCs.

Original languageEnglish
Article numbere74246
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - 2013 Sep 16

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Chemokine CXCL12
Platelets
monocytes
Monocytes
Blood Platelets
Conditioned Culture Medium
Fibronectins
cells
Cells
CXCR4 Receptors
Screening
Blood
chemokine CXCL12
fibronectins
cell differentiation
Cell Differentiation
stromal cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Platelet-Derived Stromal Cell-Derived Factor-1 Is Required for the Transformation of Circulating Monocytes into Multipotential Cells. / Seta, Noriyuki; Okazaki, Yuka; Miyazaki, Hiroshi; Kato, Takashi; Kuwana, Masataka.

In: PLoS One, Vol. 8, No. 9, e74246, 16.09.2013.

Research output: Contribution to journalArticle

Seta, Noriyuki ; Okazaki, Yuka ; Miyazaki, Hiroshi ; Kato, Takashi ; Kuwana, Masataka. / Platelet-Derived Stromal Cell-Derived Factor-1 Is Required for the Transformation of Circulating Monocytes into Multipotential Cells. In: PLoS One. 2013 ; Vol. 8, No. 9.
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