Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow

S. Goto, N. Ichikawa, M. Lee, M. Goto, H. Sakai, J. J. Kim, M. Yoshida, M. Handa, Y. Ikeda, S. Handa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background. P-selectin is known to play a crucial role in leucocyte recruitment at sites of vascular injury. Although platelet surface expression of P-selectin molecules are well known to occur after platelet stimulation by chemical agonists such as α-thrombin, it is still uncertain whether P-selectin expression occurs in the process of the more physiological platelet activation pathway mediated by interaction between von Willebrand factor (vWF) and platelet receptor proteins, including glycoprotein (GP) Ibα and GP IIb/IIIa, occurring under high shear rates generated by blood flow. Methods. We have developed a method to detect P-selectin molecules expressed on platelet surface with flow-cytometer and monoclonal antibody, which can bind exclusively to P-selectin (WGA1), directly conjugated with fluorescein isothiocynate. This method allowed us to measure platelet surface P-selectin molecules semiquantitatively. Results. We demonstrated that a significant increase in platelet surface P-selectin molecules occur after exposing platelets to a relatively high shear rate of 10,800 s-1. We have also demonstrated that shear-induced surface expression of P-selectin as well as microparticle release from platelets depended at least on the interaction between von Willebrand factor and glycoprotein Ibα, a platelet surface receptor for the former. Conclusions. Shear-induced von Willebrand-mediated surface expression of P-selectin may play a role in leucocyte recruitment in platelet thrombi at vascular injury sites.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalInternational Angiology
Volume19
Issue number2
Publication statusPublished - 2000

Fingerprint

P-Selectin
Blood Platelets
Platelet Glycoprotein GPIb-IX Complex
Vascular System Injuries
Leukocytes
Chemical Stimulation
Physiological Phenomena
Platelet Glycoprotein GPIIb-IIIa Complex
Platelet Activation
von Willebrand Factor
Fluorescein
Thrombin
Thrombosis
Monoclonal Antibodies

Keywords

  • Blood platelets
  • P-selectin
  • Von Willebrand factor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Goto, S., Ichikawa, N., Lee, M., Goto, M., Sakai, H., Kim, J. J., ... Handa, S. (2000). Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow. International Angiology, 19(2), 147-151.

Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow. / Goto, S.; Ichikawa, N.; Lee, M.; Goto, M.; Sakai, H.; Kim, J. J.; Yoshida, M.; Handa, M.; Ikeda, Y.; Handa, S.

In: International Angiology, Vol. 19, No. 2, 2000, p. 147-151.

Research output: Contribution to journalArticle

Goto, S, Ichikawa, N, Lee, M, Goto, M, Sakai, H, Kim, JJ, Yoshida, M, Handa, M, Ikeda, Y & Handa, S 2000, 'Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow', International Angiology, vol. 19, no. 2, pp. 147-151.
Goto S, Ichikawa N, Lee M, Goto M, Sakai H, Kim JJ et al. Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow. International Angiology. 2000;19(2):147-151.
Goto, S. ; Ichikawa, N. ; Lee, M. ; Goto, M. ; Sakai, H. ; Kim, J. J. ; Yoshida, M. ; Handa, M. ; Ikeda, Y. ; Handa, S. / Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow. In: International Angiology. 2000 ; Vol. 19, No. 2. pp. 147-151.
@article{052e7b70b34d43c19d2c77b7be9c80bd,
title = "Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow",
abstract = "Background. P-selectin is known to play a crucial role in leucocyte recruitment at sites of vascular injury. Although platelet surface expression of P-selectin molecules are well known to occur after platelet stimulation by chemical agonists such as α-thrombin, it is still uncertain whether P-selectin expression occurs in the process of the more physiological platelet activation pathway mediated by interaction between von Willebrand factor (vWF) and platelet receptor proteins, including glycoprotein (GP) Ibα and GP IIb/IIIa, occurring under high shear rates generated by blood flow. Methods. We have developed a method to detect P-selectin molecules expressed on platelet surface with flow-cytometer and monoclonal antibody, which can bind exclusively to P-selectin (WGA1), directly conjugated with fluorescein isothiocynate. This method allowed us to measure platelet surface P-selectin molecules semiquantitatively. Results. We demonstrated that a significant increase in platelet surface P-selectin molecules occur after exposing platelets to a relatively high shear rate of 10,800 s-1. We have also demonstrated that shear-induced surface expression of P-selectin as well as microparticle release from platelets depended at least on the interaction between von Willebrand factor and glycoprotein Ibα, a platelet surface receptor for the former. Conclusions. Shear-induced von Willebrand-mediated surface expression of P-selectin may play a role in leucocyte recruitment in platelet thrombi at vascular injury sites.",
keywords = "Blood platelets, P-selectin, Von Willebrand factor",
author = "S. Goto and N. Ichikawa and M. Lee and M. Goto and H. Sakai and Kim, {J. J.} and M. Yoshida and M. Handa and Y. Ikeda and S. Handa",
year = "2000",
language = "English",
volume = "19",
pages = "147--151",
journal = "International Angiology",
issn = "0392-9590",
publisher = "Edizioni Minerva Medica S.p.A.",
number = "2",

}

TY - JOUR

T1 - Platelet surface P-selectin molecules increased after exposing platelet to a high shear flow

AU - Goto, S.

AU - Ichikawa, N.

AU - Lee, M.

AU - Goto, M.

AU - Sakai, H.

AU - Kim, J. J.

AU - Yoshida, M.

AU - Handa, M.

AU - Ikeda, Y.

AU - Handa, S.

PY - 2000

Y1 - 2000

N2 - Background. P-selectin is known to play a crucial role in leucocyte recruitment at sites of vascular injury. Although platelet surface expression of P-selectin molecules are well known to occur after platelet stimulation by chemical agonists such as α-thrombin, it is still uncertain whether P-selectin expression occurs in the process of the more physiological platelet activation pathway mediated by interaction between von Willebrand factor (vWF) and platelet receptor proteins, including glycoprotein (GP) Ibα and GP IIb/IIIa, occurring under high shear rates generated by blood flow. Methods. We have developed a method to detect P-selectin molecules expressed on platelet surface with flow-cytometer and monoclonal antibody, which can bind exclusively to P-selectin (WGA1), directly conjugated with fluorescein isothiocynate. This method allowed us to measure platelet surface P-selectin molecules semiquantitatively. Results. We demonstrated that a significant increase in platelet surface P-selectin molecules occur after exposing platelets to a relatively high shear rate of 10,800 s-1. We have also demonstrated that shear-induced surface expression of P-selectin as well as microparticle release from platelets depended at least on the interaction between von Willebrand factor and glycoprotein Ibα, a platelet surface receptor for the former. Conclusions. Shear-induced von Willebrand-mediated surface expression of P-selectin may play a role in leucocyte recruitment in platelet thrombi at vascular injury sites.

AB - Background. P-selectin is known to play a crucial role in leucocyte recruitment at sites of vascular injury. Although platelet surface expression of P-selectin molecules are well known to occur after platelet stimulation by chemical agonists such as α-thrombin, it is still uncertain whether P-selectin expression occurs in the process of the more physiological platelet activation pathway mediated by interaction between von Willebrand factor (vWF) and platelet receptor proteins, including glycoprotein (GP) Ibα and GP IIb/IIIa, occurring under high shear rates generated by blood flow. Methods. We have developed a method to detect P-selectin molecules expressed on platelet surface with flow-cytometer and monoclonal antibody, which can bind exclusively to P-selectin (WGA1), directly conjugated with fluorescein isothiocynate. This method allowed us to measure platelet surface P-selectin molecules semiquantitatively. Results. We demonstrated that a significant increase in platelet surface P-selectin molecules occur after exposing platelets to a relatively high shear rate of 10,800 s-1. We have also demonstrated that shear-induced surface expression of P-selectin as well as microparticle release from platelets depended at least on the interaction between von Willebrand factor and glycoprotein Ibα, a platelet surface receptor for the former. Conclusions. Shear-induced von Willebrand-mediated surface expression of P-selectin may play a role in leucocyte recruitment in platelet thrombi at vascular injury sites.

KW - Blood platelets

KW - P-selectin

KW - Von Willebrand factor

UR - http://www.scopus.com/inward/record.url?scp=0034086095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034086095&partnerID=8YFLogxK

M3 - Article

C2 - 10905798

AN - SCOPUS:0034086095

VL - 19

SP - 147

EP - 151

JO - International Angiology

JF - International Angiology

SN - 0392-9590

IS - 2

ER -