Platelet unresponsiveness to collagen: Involvement of glycoprotein Ia-IIa (α2β1 integrin) deficiency associated with a myeloproliferative disorder

M. Handa, K. Watanabe, Y. Kawai, Tetsuji Kamata, T. Koyama, H. Nagai, Y. Ikeda

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Abstract

We studied a 66-year-old man with a myeloproliferative disorder who presented with a prolonged bleeding time and marked thrombocytosis (platelet count, 3,890 x 109/l). There was no past history of a bleeding disorder. The patient had normal coagulation data. His platelets completely lacked collagen-induced platelet aggregation and adhesion, but showed normal responses to other agonists. All family members tested showed normal platelet aggregation with collagen. Analysis of 125I surface-labeled platelets by two-dimensional SDS gel electrophoresis disclosed absence of the spot corresponding to platelet membrane GPIa (α2) but no other significant deficiencies of major platelet glycoproteins i.e., GPIb, IIb-IIIa, and IV. Immunoisolation studies of the patient's platelets indicated that neither anti-GPIa nor anti-GPIIa (β1) monoclonal antibody (mAb) isolated any surface membrane proteins corresponding to GPIa. GPVI, a putative collagen receptor, was immunoisolated from the platelets. Indirect immunofluorescence study using flow cytometry confirmed that the patient's platelets were totally deficient in surface expression of the GPIa-IIa complex (α2β1 integrin). In contrast, phytohemoagglutinin-activated T-lymphocytes from the patient expressed normal concentrations of this complex. The data suggest that our patient had an acquired deficiency of the platelet GPIa-IIa complex, due to a myeloproliferative disorder, which might account for the absence of responsiveness of his platelet to collagen.

Original languageEnglish
Pages (from-to)521-528
Number of pages8
JournalThrombosis and Haemostasis
Volume73
Issue number3
Publication statusPublished - 1995

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Integrin alpha2beta1
Myeloproliferative Disorders
Integrin alpha2
Integrins
Collagen
Blood Platelets
Platelet Aggregation
Membrane Proteins
Collagen Receptors
Platelet Membrane Glycoproteins
Thrombocytosis
Bleeding Time
Electrophoresis, Gel, Two-Dimensional
Indirect Fluorescent Antibody Technique
Platelet Count
Flow Cytometry
Monoclonal Antibodies
Hemorrhage
T-Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Platelet unresponsiveness to collagen : Involvement of glycoprotein Ia-IIa (α2β1 integrin) deficiency associated with a myeloproliferative disorder. / Handa, M.; Watanabe, K.; Kawai, Y.; Kamata, Tetsuji; Koyama, T.; Nagai, H.; Ikeda, Y.

In: Thrombosis and Haemostasis, Vol. 73, No. 3, 1995, p. 521-528.

Research output: Contribution to journalArticle

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