TY - JOUR
T1 - PM2.5 collected using cyclonic separation causes stronger biological responses than that collected using a conventional filtration method
AU - Honda, Akiko
AU - Okuda, Tomoaki
AU - Nagao, Megumi
AU - Miyasaka, Natsuko
AU - Tanaka, Michitaka
AU - Takano, Hirohisa
N1 - Funding Information:
This study was supported by the Environmental Research and Technology Development Fund ( 5–1651 ) of the Ministry of the Environment , Japan. We thank Shin Tamura, Wang Zaoshi, Eiji Kawasaki, Kiyoe Itoi, Yufuko Kobayashi for their technical assistance.
PY - 2020
Y1 - 2020
N2 - Evaluation of the health effects of particulate matter with aerodynamic dias. ≤ 2.5 μm (PM2.5) should reflect realistic condition in ambient atmosphere. However, using conventional filtration methods, only extracts from PM2.5 collected on the filter can be analyzed and not the particle itself. Cyclonic separation is a technique that enables the direct analysis of the effects of the crude “powder form” of PM2.5 on respiratory health. Airway epithelial cells and antigen-presenting cells were exposed to PM2.5 collected during the same period using a conventional filtration method or cyclonic separation. PM2.5 collected using cyclonic separation led to a higher secretion of interleukins 6 and 8 (IL-6, IL-8) from airway epithelial cells, and IL-6, IL-1β, tumor necrosis factor-α (TNF-α) secretion, cluster of differentiation 86 (CD86), and dendritic and epithelial cells 205 (DEC205) expression on antigen-presenting cells, compared with the effects of filter-collected PM2.5. Furthermore, PM2.5 collected using cyclonic separation increased inflammatory cytokine levels and induced lung inflammation in vivo. These results suggest that crude PM2.5 collected using cyclonic separation causes stronger biological responses than filter-collected PM2.5. Hence, PM2.5 collected using cyclonic separation can be utilized for a reliable evaluation of the health effects of ambient PM2.5.
AB - Evaluation of the health effects of particulate matter with aerodynamic dias. ≤ 2.5 μm (PM2.5) should reflect realistic condition in ambient atmosphere. However, using conventional filtration methods, only extracts from PM2.5 collected on the filter can be analyzed and not the particle itself. Cyclonic separation is a technique that enables the direct analysis of the effects of the crude “powder form” of PM2.5 on respiratory health. Airway epithelial cells and antigen-presenting cells were exposed to PM2.5 collected during the same period using a conventional filtration method or cyclonic separation. PM2.5 collected using cyclonic separation led to a higher secretion of interleukins 6 and 8 (IL-6, IL-8) from airway epithelial cells, and IL-6, IL-1β, tumor necrosis factor-α (TNF-α) secretion, cluster of differentiation 86 (CD86), and dendritic and epithelial cells 205 (DEC205) expression on antigen-presenting cells, compared with the effects of filter-collected PM2.5. Furthermore, PM2.5 collected using cyclonic separation increased inflammatory cytokine levels and induced lung inflammation in vivo. These results suggest that crude PM2.5 collected using cyclonic separation causes stronger biological responses than filter-collected PM2.5. Hence, PM2.5 collected using cyclonic separation can be utilized for a reliable evaluation of the health effects of ambient PM2.5.
KW - Crude PM2.5
KW - Cyclone
KW - Filter
KW - Immune system
KW - Respiratory system
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U2 - 10.1016/j.envres.2020.110490
DO - 10.1016/j.envres.2020.110490
M3 - Article
C2 - 33220242
AN - SCOPUS:85097050739
JO - Environmental Research
JF - Environmental Research
SN - 0013-9351
M1 - 110490
ER -