PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis

Tetsuya Hirabayashi, Tatsuki Anjo, Arisa Kaneko, Yuuya Senoo, Akitaka Shibata, Hiroyuki Takama, Kohei Yokoyama, Yasumasa Nishito, Tomio Ono, Choji Taya, Kazuaki Muramatsu, Kiyoko Fukami, Agustí Muñoz-Garcia, Alan R. Brash, Kazutaka Ikeda, Makoto Arita, Masashi Akiyama, Makoto Murakami

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Mutations in patatin-like phospholipase domain-containing 1 (PNPLA1) cause autosomal recessive congenital ichthyosis, but the mechanism involved remains unclear. Here we show that PNPLA1, an enzyme expressed in differentiated keratinocytes, plays a crucial role in the biosynthesis of ω-O-acylceramide, a lipid component essential for skin barrier. Global or keratinocyte-specific Pnpla1-deficient neonates die due to epidermal permeability barrier defects with severe transepidermal water loss, decreased intercellular lipid lamellae in the stratum corneum, and aberrant keratinocyte differentiation. In Pnpla1-/- epidermis, unique linoleate-containing lipids including acylceramides, acylglucosylceramides and (O-acyl)-ω-hydroxy fatty acids are almost absent with reciprocal increases in their putative precursors, indicating that PNPLA1 catalyses the ω-O-esterification with linoleic acid to form acylceramides. Moreover, acylceramide supplementation partially rescues the altered differentiation of Pnpla1-/- keratinocytes. Our findings provide valuable insight into the skin barrier formation and ichthyosis development, and may contribute to novel therapeutic strategies for treatment of epidermal barrier defects.

Original languageEnglish
Article number14609
JournalNature Communications
Volume8
DOIs
Publication statusPublished - 2017 Mar 1
Externally publishedYes

Fingerprint

biosynthesis
Phospholipases
Biosynthesis
Keratinocytes
Skin
Ichthyosis
lipids
Linoleic Acid
Lipids
water loss
epidermis
Defects
Hydroxy Acids
cornea
Esterification
defects
fatty acids
strata
lamella
mutations

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Hirabayashi, T., Anjo, T., Kaneko, A., Senoo, Y., Shibata, A., Takama, H., ... Murakami, M. (2017). PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis. Nature Communications, 8, [14609]. https://doi.org/10.1038/ncomms14609

PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis. / Hirabayashi, Tetsuya; Anjo, Tatsuki; Kaneko, Arisa; Senoo, Yuuya; Shibata, Akitaka; Takama, Hiroyuki; Yokoyama, Kohei; Nishito, Yasumasa; Ono, Tomio; Taya, Choji; Muramatsu, Kazuaki; Fukami, Kiyoko; Muñoz-Garcia, Agustí; Brash, Alan R.; Ikeda, Kazutaka; Arita, Makoto; Akiyama, Masashi; Murakami, Makoto.

In: Nature Communications, Vol. 8, 14609, 01.03.2017.

Research output: Contribution to journalArticle

Hirabayashi, T, Anjo, T, Kaneko, A, Senoo, Y, Shibata, A, Takama, H, Yokoyama, K, Nishito, Y, Ono, T, Taya, C, Muramatsu, K, Fukami, K, Muñoz-Garcia, A, Brash, AR, Ikeda, K, Arita, M, Akiyama, M & Murakami, M 2017, 'PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis', Nature Communications, vol. 8, 14609. https://doi.org/10.1038/ncomms14609
Hirabayashi, Tetsuya ; Anjo, Tatsuki ; Kaneko, Arisa ; Senoo, Yuuya ; Shibata, Akitaka ; Takama, Hiroyuki ; Yokoyama, Kohei ; Nishito, Yasumasa ; Ono, Tomio ; Taya, Choji ; Muramatsu, Kazuaki ; Fukami, Kiyoko ; Muñoz-Garcia, Agustí ; Brash, Alan R. ; Ikeda, Kazutaka ; Arita, Makoto ; Akiyama, Masashi ; Murakami, Makoto. / PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis. In: Nature Communications. 2017 ; Vol. 8.
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