Polaprezinc, a gastroprotective agent: Attenuation of monochloramine-evoked gastric DNA fragmentation

Hidekazu Suzuki, Mikiji Mori, Koichi Seto, Shoichi Nagahashi, Chizuko Kawaguchi, Hitoshi Morita, Masayuki Suzuki, Soichiro Miura, Tomoyuki Yoneta, Hiromasa Ishii

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We previously reported that NH2Cl induced extensive DNA fragmentation in gastric cells. Polaprezinc, a zinc-carnosine chelate compound, is reported to be a potent antioxidant in gastric mucosa. The present study was designed to examine whether polaprezinc could attenuate the NH2Cl-induced DNA damage. Gastric cell lines, MKN45, were exposed to NH2Cl in Ca2+-containing Hanks' balanced salt solution. DNA fragmentation was evaluated by photometric enzyme immunoassay for in vitro determination of cytoplasmic mono- and oligonucleosomes. Polaprezinc, L-carnosine, and zinc sulfate (ZnSO4) were added to the cell incubation medium to evaluate the inhibitory effect on the formation of cytoplasmic mono- and oligonucleosomes. Separately, the bleaching level of β-carotene with the addition of each test solution was evaluated to confirm the inhibitory effect against hypochlorous acid. Polaprezinc or L-carnosine, but not ZnSO4, at a concentration of 0.001 mM, significantly attenuated the increased levels of cytoplasmic mono- and oligonucleosomes evoked by 0.001 mM NH2Cl. Polaprezinc and L-carnosine, but not ZnSO4, also inhibited NH2Cl-induced β-carotene bleaching in the cell-free system. In conclusion, polaprezinc, especially its subportion L-carnosine, inhibited NH2Cl-evoked gastric epithelial DNA fragmentation, suggesting a role for this agent in preventing the progression of gastric epithelial injury induced by NH2Cl.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalJournal of gastroenterology
Volume34
Issue numberSUPPL. 11
Publication statusPublished - 1999 Dec 1

Keywords

  • Apoptosis
  • Helicobacter pylori
  • L-carnosine
  • Leukocyte
  • Urease
  • Zinc sulfate

ASJC Scopus subject areas

  • Gastroenterology

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