Polaprezinc, a gastroprotective agent: Attenuation of monochloramine-evoked gastric DNA fragmentation

Hidekazu Suzuki, Mikiji Mori, Koichi Seto, Shoichi Nagahashi, Chizuko Kawaguchi, Hitoshi Morita, Masayuki Suzuki, Soichiro Miura, Tomoyuki Yoneta, Hiromasa Ishii

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We previously reported that NH2Cl induced extensive DNA fragmentation in gastric cells. Polaprezinc, a zinc-carnosine chelate compound, is reported to be a potent antioxidant in gastric mucosa. The present study was designed to examine whether polaprezinc could attenuate the NH2Cl-induced DNA damage. Gastric cell lines, MKN45, were exposed to NH2Cl in Ca2+-containing Hanks' balanced salt solution. DNA fragmentation was evaluated by photometric enzyme immunoassay for in vitro determination of cytoplasmic mono- and oligonucleosomes. Polaprezinc, L-carnosine, and zinc sulfate (ZnSO4) were added to the cell incubation medium to evaluate the inhibitory effect on the formation of cytoplasmic mono- and oligonucleosomes. Separately, the bleaching level of β-carotene with the addition of each test solution was evaluated to confirm the inhibitory effect against hypochlorous acid. Polaprezinc or L-carnosine, but not ZnSO4, at a concentration of 0.001 mM, significantly attenuated the increased levels of cytoplasmic mono- and oligonucleosomes evoked by 0.001 mM NH2Cl. Polaprezinc and L-carnosine, but not ZnSO4, also inhibited NH2Cl-induced β-carotene bleaching in the cell-free system. In conclusion, polaprezinc, especially its subportion L-carnosine, inhibited NH2Cl-evoked gastric epithelial DNA fragmentation, suggesting a role for this agent in preventing the progression of gastric epithelial injury induced by NH2Cl.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalJournal of Gastroenterology
Volume34
Issue numberSUPPL. 11
Publication statusPublished - 1999 Dec

Fingerprint

polaprezinc
Carnosine
DNA Fragmentation
Stomach
Carotenoids
Zinc Sulfate
Hypochlorous Acid
Cell-Free System
Gastric Mucosa
Immunoenzyme Techniques
DNA Damage
chloramine
Zinc
Antioxidants
Cell Line

Keywords

  • Apoptosis
  • Helicobacter pylori
  • L-carnosine
  • Leukocyte
  • Urease
  • Zinc sulfate

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Suzuki, H., Mori, M., Seto, K., Nagahashi, S., Kawaguchi, C., Morita, H., ... Ishii, H. (1999). Polaprezinc, a gastroprotective agent: Attenuation of monochloramine-evoked gastric DNA fragmentation. Journal of Gastroenterology, 34(SUPPL. 11), 43-46.

Polaprezinc, a gastroprotective agent : Attenuation of monochloramine-evoked gastric DNA fragmentation. / Suzuki, Hidekazu; Mori, Mikiji; Seto, Koichi; Nagahashi, Shoichi; Kawaguchi, Chizuko; Morita, Hitoshi; Suzuki, Masayuki; Miura, Soichiro; Yoneta, Tomoyuki; Ishii, Hiromasa.

In: Journal of Gastroenterology, Vol. 34, No. SUPPL. 11, 12.1999, p. 43-46.

Research output: Contribution to journalArticle

Suzuki, H, Mori, M, Seto, K, Nagahashi, S, Kawaguchi, C, Morita, H, Suzuki, M, Miura, S, Yoneta, T & Ishii, H 1999, 'Polaprezinc, a gastroprotective agent: Attenuation of monochloramine-evoked gastric DNA fragmentation', Journal of Gastroenterology, vol. 34, no. SUPPL. 11, pp. 43-46.
Suzuki H, Mori M, Seto K, Nagahashi S, Kawaguchi C, Morita H et al. Polaprezinc, a gastroprotective agent: Attenuation of monochloramine-evoked gastric DNA fragmentation. Journal of Gastroenterology. 1999 Dec;34(SUPPL. 11):43-46.
Suzuki, Hidekazu ; Mori, Mikiji ; Seto, Koichi ; Nagahashi, Shoichi ; Kawaguchi, Chizuko ; Morita, Hitoshi ; Suzuki, Masayuki ; Miura, Soichiro ; Yoneta, Tomoyuki ; Ishii, Hiromasa. / Polaprezinc, a gastroprotective agent : Attenuation of monochloramine-evoked gastric DNA fragmentation. In: Journal of Gastroenterology. 1999 ; Vol. 34, No. SUPPL. 11. pp. 43-46.
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