Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis

Y. Tamura, Siro Simizu, M. Muroi, S. Takagi, M. Kawatani, N. Watanabe, H. Osada

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bcl-xL, an anti-apoptotic Bcl-2 family member protein, contributes to the resistance against chemotherapies such as tubulin-binder treatment in many human tumors. Although Bcl-xL is phosphorylated after tubulin-binder treatment, the role of the phosphorylation and its responsible kinase(s) are poorly understood. Here, we identified Plk1 (polo-like kinase 1) as a Bcl-xL kinase. Same location of Bcl-xL and Plk1 was revealed by immunocytochemical analyses at M-phase in situ. Plk1 phosphorylates Bcl-xL in vitro, and we identified Plk1 phosphorylation sites in Bcl-xL. When all of these phosphorylation sites were substituted to alanines, the anti-apoptotic activity of the Bcl-xL mutant against the apoptosis induced by pironetin, but not against ultraviolet-induced apoptosis, was increased. These observations suggest that Plk1 is a regulator of Bcl-xL phosphorylation and controls the anti-apoptotic activity of Bcl-xL during pironetin-induced apoptosis.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalOncogene
Volume28
Issue number1
DOIs
Publication statusPublished - 2009 Jan 8
Externally publishedYes

Fingerprint

Apoptosis
Phosphorylation
Tubulin
Phosphotransferases
Alanine
Cell Division
pironetin
polo-like kinase 1
Drug Therapy
Therapeutics
Neoplasms
Proteins

Keywords

  • Apoptosis
  • Bcl-x
  • Phosphorylation
  • Pironetin
  • Plk1

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Tamura, Y., Simizu, S., Muroi, M., Takagi, S., Kawatani, M., Watanabe, N., & Osada, H. (2009). Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis. Oncogene, 28(1), 107-116. https://doi.org/10.1038/onc.2008.368

Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis. / Tamura, Y.; Simizu, Siro; Muroi, M.; Takagi, S.; Kawatani, M.; Watanabe, N.; Osada, H.

In: Oncogene, Vol. 28, No. 1, 08.01.2009, p. 107-116.

Research output: Contribution to journalArticle

Tamura, Y, Simizu, S, Muroi, M, Takagi, S, Kawatani, M, Watanabe, N & Osada, H 2009, 'Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis', Oncogene, vol. 28, no. 1, pp. 107-116. https://doi.org/10.1038/onc.2008.368
Tamura, Y. ; Simizu, Siro ; Muroi, M. ; Takagi, S. ; Kawatani, M. ; Watanabe, N. ; Osada, H. / Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis. In: Oncogene. 2009 ; Vol. 28, No. 1. pp. 107-116.
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