Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturation

Yuko Ozaki, Hirotaka Matsui, Hiroya Asou, Akiko Nagamachi, Daisuke Aki, Hiroaki Honda, Shin'ichiro Yasunaga, Yoshihiro Takihara, Tadashi Yamamoto, Shunsuke Izumi, Miho Ohsugi, Toshiya Inaba

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

During prometaphase, dense microtubule nucleation sites at centrosomes form robust spindles that align chromosomes promptly. Failure of centrosome maturation leaves chromosomes scattered, as seen routinely in cancer cells, including myelodysplastic syndrome (MDS). We previously reported that the Miki (LOC253012) gene is frequently deleted in MDS patients, and that low levels of Miki are associated with abnormal mitosis. Here we demonstrate that Miki localizes to the Golgi apparatus and is poly(ADP-ribosyl)ated by tankyrase-1 during late G2 and prophase. PARsylated Miki then translocates to mitotic centrosomes and anchors CG-NAP, a large scaffold protein of the γ-tubulin ring complex. Due to impairment of microtubule aster formation, cells in which tankyrase-1, Miki, or CG-NAP expression is downregulated all show prometaphase disturbances, including scattered and lagging chromosomes. Our data suggest that PARsylation of Miki by tankyrase-1 is a key initial event promoting prometaphase.

Original languageEnglish
Pages (from-to)694-706
Number of pages13
JournalMolecular Cell
Volume47
Issue number5
DOIs
Publication statusPublished - 2012 Sep 14
Externally publishedYes

Fingerprint

Tankyrases
Prometaphase
Centrosome
Adenosine Diphosphate
Chromosomes
Myelodysplastic Syndromes
Microtubules
Prophase
Golgi Apparatus
Tubulin
Mitosis
Down-Regulation
Genes
Neoplasms
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Ozaki, Y., Matsui, H., Asou, H., Nagamachi, A., Aki, D., Honda, H., ... Inaba, T. (2012). Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturation. Molecular Cell, 47(5), 694-706. https://doi.org/10.1016/j.molcel.2012.06.033

Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturation. / Ozaki, Yuko; Matsui, Hirotaka; Asou, Hiroya; Nagamachi, Akiko; Aki, Daisuke; Honda, Hiroaki; Yasunaga, Shin'ichiro; Takihara, Yoshihiro; Yamamoto, Tadashi; Izumi, Shunsuke; Ohsugi, Miho; Inaba, Toshiya.

In: Molecular Cell, Vol. 47, No. 5, 14.09.2012, p. 694-706.

Research output: Contribution to journalArticle

Ozaki, Y, Matsui, H, Asou, H, Nagamachi, A, Aki, D, Honda, H, Yasunaga, S, Takihara, Y, Yamamoto, T, Izumi, S, Ohsugi, M & Inaba, T 2012, 'Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturation', Molecular Cell, vol. 47, no. 5, pp. 694-706. https://doi.org/10.1016/j.molcel.2012.06.033
Ozaki, Yuko ; Matsui, Hirotaka ; Asou, Hiroya ; Nagamachi, Akiko ; Aki, Daisuke ; Honda, Hiroaki ; Yasunaga, Shin'ichiro ; Takihara, Yoshihiro ; Yamamoto, Tadashi ; Izumi, Shunsuke ; Ohsugi, Miho ; Inaba, Toshiya. / Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturation. In: Molecular Cell. 2012 ; Vol. 47, No. 5. pp. 694-706.
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