Poly-dipeptides encoded by the C9ORF72 repeats block global protein translation

Kohsuke Kanekura, Takuya Yagi, Alexander J. Cammack, Jana Mahadevan, Masahiko Kuroda, Matthew B. Harms, Timothy M. Miller, Fumihiko Urano

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Abstract

The expansion of the GGGGCC hexanucleotide repeat in the non-coding region of the Chromosome 9 open-reading frame 72 (C9orf72) gene is the most common genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). This genetic alteration leads to the accumulation of five types of poly-dipeptides translated from the GGGGCC hexanucleotide repeat. Among these, poly-proline-arginine (poly-PR) and poly-glycine-arginine (poly-GR) peptides are known to be neurotoxic. However, the mechanisms of neurotoxicity associated with these poly-dipeptides are not clear. A proteomics approach identified a number of interacting proteins with poly-PR peptide, including mRNA-binding proteins, ribosomal proteins, translation initiation factors and translation elongation factors. Immunostaining of brain sections from patients with C9orf72 ALS showed that poly-GR was colocalized with a mRNA-binding protein, hnRNPA1. In vitro translation assays showed that poly-PR and poly-GR peptides made insoluble complexes with mRNA, restrained the access of translation factors to mRNA, and blocked protein translation. Our results demonstrate that impaired protein translation mediated by poly-PR and poly-GR peptides plays a role in neurotoxicity and reveal that the pathways altered by the poly-dipeptides-mRNA complexes are potential therapeutic targets for treatment of C9orf72 FTD/ALS.

Original languageEnglish
Article numberddw052
Pages (from-to)1803-1813
Number of pages11
JournalHuman Molecular Genetics
Volume25
Issue number9
DOIs
Publication statusPublished - 2016 May 1
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Kanekura, K., Yagi, T., Cammack, A. J., Mahadevan, J., Kuroda, M., Harms, M. B., Miller, T. M., & Urano, F. (2016). Poly-dipeptides encoded by the C9ORF72 repeats block global protein translation. Human Molecular Genetics, 25(9), 1803-1813. [ddw052]. https://doi.org/10.1093/hmg/ddw052