Polyclonal uses of T-cell receptor (TCR)α and β genes for cytotoxic T lymphocytes in human metastatic melanoma

Possible involvement of TCRα in tumor-cell recognition

D. Seito, T. Morita, K. Masuoka, T. Maeda, Hideyuki Saya, K. Itoh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Identification of genetic structure and diversity of T-cell receptor (TCR)α and β genes for cytotoxic T lymphocytes (CTLs) infiltrating human cancers is important for the better understanding of molecular mechanisms of host defense at tumor sites. cDNAs of TCRα and β genes of 22 different melanoma-specific CTL clones established from the tumor-infiltrating lymphocytes of 2 patients were sequenced for analysis of their genetic structure and diversity. Vα7.2-Jα10-Cα was found in 4 of 22 clones, 2 of which also used the same β-chain. The other 20 clones showed different combinations of α and β use. At deduced amino-acid levels, 7 of 9 clones from one patient used a threonine residue at the 26th position in the complementarity-determining region (CDR) 1 of TCRα. Eight of 13 clones used a threonine at the 99th or a serine residue at the 100th position in CDR3 of TCRα. CTL clones with the same or different TCRαα showed the same or different patterns of cytotoxicity, respectively. These results suggest that CTLs usually do not demonstrate clonal expansion at tumor sites of metastatic melanoma's but rather that polyclonal T cells capable of binding to multiple melanoma determinants through CDR3 of TCRα accumulate in the tumor.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalInternational Journal of Cancer
Volume58
Issue number4
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

T-Cell Receptor Genes
Cytotoxic T-Lymphocytes
T-Cell Antigen Receptor
Melanoma
Clone Cells
Neoplasms
Genetic Structures
Threonine
Complementarity Determining Regions
Tumor-Infiltrating Lymphocytes
Serine
Complementary DNA
T-Lymphocytes
Amino Acids

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Polyclonal uses of T-cell receptor (TCR)α and β genes for cytotoxic T lymphocytes in human metastatic melanoma : Possible involvement of TCRα in tumor-cell recognition. / Seito, D.; Morita, T.; Masuoka, K.; Maeda, T.; Saya, Hideyuki; Itoh, K.

In: International Journal of Cancer, Vol. 58, No. 4, 1994, p. 497-502.

Research output: Contribution to journalArticle

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