Polymer particles as the carrier for affinity purification

Mamoru Hatakeyama, Kosuke Nishio, Mitsuhiro Nakamura, Satoshi Sakamoto, Yasuaki Kabe, Tadashi Wada, Hiroshi Handa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Affinity purification of biomolecules which is essential for the breakthrough of biological science, can be assayed by using gel columns. However, the target materials obtained by the gel column method did not obtain high purity and the capture process wasted much time. So we constructed the non-porus particles which had large surface areas by soap-free emulsion polymerization for affinity purification. The diameter of obtained particles was about 200 nm. Then we immobilized the DNAs, peptides, proteins and biomimetic substrates such as the drugs onto these particles and purified the target proteins bound to these particles. We picked up some significant topics about affinity purification. We also report the preparation of ferrite-polymer hybrid nanoparticles for magnetic separation and the affinity purification by using the polymer nanoparticles included in ferrite.

Original languageEnglish
Pages (from-to)9-20
Number of pages12
JournalKobunshi Ronbunshu
Volume64
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1
Externally publishedYes

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Keywords

  • Affinity purification
  • Automation
  • Emulsion polymerization
  • Epoxy group
  • Ferrite
  • Glycigyl methacrylate
  • Immobilization of ligands
  • Magnetic separation
  • Nanoparticles
  • Nonspecific adsorption

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Materials Science (miscellaneous)
  • Environmental Science(all)
  • Polymers and Plastics

Cite this

Hatakeyama, M., Nishio, K., Nakamura, M., Sakamoto, S., Kabe, Y., Wada, T., & Handa, H. (2007). Polymer particles as the carrier for affinity purification. Kobunshi Ronbunshu, 64(1), 9-20. https://doi.org/10.1295/koron.64.9