Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor

Noriko Yoneda, Satoshi Yoneda, Hideki Niimi, Tomohiro Ueno, Shirou Hayashi, Mika Ito, Arihiro Shiozaki, Daichi Urushiyama, Kenichiro Hata, Wataru Suda, Masahira Hattori, Mika Kigawa, Isao Kitajima, Shigeru Saito

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Problem: To study the relationship between perinatal prognosis in cases of preterm labor (PTL) and polymicrobial infection in amniotic fluid (AF) and intra-amniotic (IA) inflammation using a highly sensitive and reliable PCR-based method. Method of Study: To detect prokaryotes using a nested PCR-based method, eukaryote-made thermostable DNA polymerase without bacterial DNA contamination was used in combination with bacterial universal primers. We collected AF aseptically from 118 PTL cases and 50 term subjects. Results: The prevalence of microorganisms was 33% (39/118) by PCR and only 7.6% (9/118) by culture. PTL caused by a combination of positive Mycoplasma/Ureaplasma and other bacteria had significantly higher AF IL-8 levels and a significantly shorter amniocentesis-to-delivery interval. Conclusions: Our newly established PCR method is useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in PTL.

Original languageEnglish
Pages (from-to)112-125
Number of pages14
JournalAmerican Journal of Reproductive Immunology
Volume75
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1
Externally publishedYes

Fingerprint

Ureaplasma
Mycoplasma Infections
Premature Obstetric Labor
Amniotic Fluid
Inflammation
Bacteria
Polymerase Chain Reaction
Mycoplasma
Coinfection
DNA Contamination
Bacterial DNA
Amniocentesis
DNA-Directed DNA Polymerase
Eukaryota
Interleukin-8

Keywords

  • Eukaryote-made thermostable DNA polymerase
  • Intra-amniotic infection
  • Polymerase chain reaction
  • Polymicrobial infection
  • Preterm labor
  • Ureaplasma spp

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor. / Yoneda, Noriko; Yoneda, Satoshi; Niimi, Hideki; Ueno, Tomohiro; Hayashi, Shirou; Ito, Mika; Shiozaki, Arihiro; Urushiyama, Daichi; Hata, Kenichiro; Suda, Wataru; Hattori, Masahira; Kigawa, Mika; Kitajima, Isao; Saito, Shigeru.

In: American Journal of Reproductive Immunology, Vol. 75, No. 2, 01.02.2016, p. 112-125.

Research output: Contribution to journalArticle

Yoneda, N, Yoneda, S, Niimi, H, Ueno, T, Hayashi, S, Ito, M, Shiozaki, A, Urushiyama, D, Hata, K, Suda, W, Hattori, M, Kigawa, M, Kitajima, I & Saito, S 2016, 'Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor', American Journal of Reproductive Immunology, vol. 75, no. 2, pp. 112-125. https://doi.org/10.1111/aji.12456
Yoneda, Noriko ; Yoneda, Satoshi ; Niimi, Hideki ; Ueno, Tomohiro ; Hayashi, Shirou ; Ito, Mika ; Shiozaki, Arihiro ; Urushiyama, Daichi ; Hata, Kenichiro ; Suda, Wataru ; Hattori, Masahira ; Kigawa, Mika ; Kitajima, Isao ; Saito, Shigeru. / Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor. In: American Journal of Reproductive Immunology. 2016 ; Vol. 75, No. 2. pp. 112-125.
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