Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation

Masaki Shibayama, Satona Ohno, Takashi Osaka, Reiko Sakamoto, Akinori Tokunaga, Yuki Nakatake, Mitsuharu Sato, Nobuaki Yoshida

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Polypyrimidine tract-binding protein (PTB) is a widely expressed RNA-binding protein with multiple roles in RNA processing, including the splicing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site-dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear. Here, we report that a homozygous mutation in the mouse Ptb gene causes embryonic lethality shortly after implantation. We also established Ptb-/- embryonic stem (ES) cell lines and found that these mutant cells exhibited severe defects in cell proliferation without aberrant differentiation in vitro or in vivo. Furthermore, cell cycle analysis and a cell synchronization assay revealed that Ptb -/- ES cells have a prolonged G2/M phase. Thus, our data indicate that PTB is essential for early mouse development and ES cell proliferation.

Original languageEnglish
Pages (from-to)6658-6668
Number of pages11
JournalFEBS Journal
Volume276
Issue number22
DOIs
Publication statusPublished - 2009 Nov
Externally publishedYes

Fingerprint

Polypyrimidine Tract-Binding Protein
Cell proliferation
Stem cells
Cell Proliferation
Embryonic Stem Cells
Messenger RNA
RNA-Binding Proteins
G2 Phase
RNA Stability
Alternative Splicing
Cell growth
Cell Division
Exons
Assays
Cell Cycle
Synchronization
Genes
Cells
RNA
Cell Line

Keywords

  • Cell cycle
  • Embryonic stem cells
  • Knockout mouse
  • Polypyrimidine tract-binding protein
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation. / Shibayama, Masaki; Ohno, Satona; Osaka, Takashi; Sakamoto, Reiko; Tokunaga, Akinori; Nakatake, Yuki; Sato, Mitsuharu; Yoshida, Nobuaki.

In: FEBS Journal, Vol. 276, No. 22, 11.2009, p. 6658-6668.

Research output: Contribution to journalArticle

Shibayama, Masaki ; Ohno, Satona ; Osaka, Takashi ; Sakamoto, Reiko ; Tokunaga, Akinori ; Nakatake, Yuki ; Sato, Mitsuharu ; Yoshida, Nobuaki. / Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation. In: FEBS Journal. 2009 ; Vol. 276, No. 22. pp. 6658-6668.
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